Characterization of SET/I2PP2A Isoforms in Dogs

• YABE Ryotaro

  Laboratory of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753–8515, Japan

• FUJIWARA Nobuyuki

  Laboratory of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753–8515, Japan

• MIZUNO Takuya

  Laboratory of Veterinary Clinical Pathology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753–8515, Japan

• USUI Tatsuya

  Laboratory of Veterinary Toxicology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753–8515, Japan

• OHAMA Takashi

  Laboratory of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753–8515, Japan

• SATO Koichi

  Laboratory of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753–8515, Japan

Bibliographic Information

Other Title

• Pharmacology : Characterization of SET/I2PP2A Isoforms in Dogs

Abstract

SET is an endogenous protein phosphatase 2A (PP2A) inhibitor and is associated with a poor prognosis in human leukemia. Previously, we reported increased SET protein levels in canine lymphoma cell lines and the potential therapeutic application of SET antagonists in canine lymphoma. Here, we found that canine cells express several isoforms of the SET protein. We cloned 4 isoforms of SET, named SETα, β, γ and δ. Genomic BLAST showed that the SET genes are located on chromosomes X, 7, 1 and 8, respectively. An immunofluorescent study showed nuclear localization of SETα and β, and nuclear and cytosolic localization of SETγ and δ. We confirmed that SETα and β possess the ability to associate with PP2A. Our data reveal the existence of unique SET isoforms that should be taken into account in SET-targeting drug development studies in dogs.

Journal

• Journal of Veterinary Medical Science

  Journal of Veterinary Medical Science 76 (9), 1235-1240, 2014

  JAPANESE SOCIETY OF VETERINARY SCIENCE

Keywords

• cancer
• canine
• phosphorylation
• protein phosphatase 2A
• SET/I2PP2A

Details

• CRID: 1390282681406559360
• NII Article ID: 130004432203
• NII Book ID: AA10796138
• DOI: 10.1292/jvms.14-0209
• COI: 1:STN:280:DC%2BC2cjptFOmuw%3D%3D
• ISSN: 13477439
• ISSN: 09167250
• NDL BIB ID: 025818487
• PubMed: 24897959
• Web Site: https://petit.lib.yamaguchi-u.ac.jp/24566
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025818487
• Web Site: https://www.jstage.jst.go.jp/article/jvms/76/9/76_14-0209/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015051891
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed