EMS mutagenesis and characterization of Brassica rapa mutants

DOI Web Site 参考文献19件 オープンアクセス
  • Mizuno Yosuke
    Department of Life Science, International Christian University (ICU)
  • Okamoto Shunsuke
    Plant Breeding & Experiment Station, Takii & Co., Ltd.
  • Hara Miyuki
    Department of Life Science, International Christian University (ICU) Gene Research Center, University of Tsukuba
  • Mizoguchi Tsuyoshi
    Department of Life Science, International Christian University (ICU) Gene Research Center, University of Tsukuba

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タイトル別名
  • EMS mutagenesis and characterization of <i>Brassica rapa</i> mutants

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Brassica rapa is an important model crop in the genus Brassica, which includes various important vegetable crops such as Chinese cabbage and turnip, and is closely related to Arabidopsis thaliana (Arabidopsis). B. rapa var. trilocularis (yellow sarson), a rapid-cycling population of B. rapa, is commonly used for genetic research and is expected to bridge the gap between the model plant Arabidopsis and Brassica crops. In this study, 940 M2 mutagenized lines of yellow sarson were produced using ethylmethanesulfonate (EMS) mutagenesis, and these M2 plants were examined for alterations in visible phenotypes. In total, 293 independent M2 lines (including 2188 M2 plants) were investigated for phenotypic alterations, and 394 individual mutants were isolated. Subsequently, the observed mutant phenotypes were classified into 8 major categories and 18 subcategories. In addition, three mutants, namely, early flowering 1 (elf1), crane-like 1 (crl1), and rosette 2 (ros2), were selected for further phenotypic and genetic analysis. elf1 and ros2 showed early and late flowering phenotypes, respectively. Based on their phenotypes, crl1 and ros2 are likely mutations in genes for auxin signaling and gibberellic acid biosynthesis, respectively.

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