EMS mutagenesis and characterization of <i>Brassica rapa</i> mutants

• Mizuno Yosuke

  Department of Life Science, International Christian University (ICU)

• Okamoto Shunsuke

  Plant Breeding & Experiment Station, Takii & Co., Ltd.

• Hara Miyuki

  Department of Life Science, International Christian University (ICU) Gene Research Center, University of Tsukuba

• Mizoguchi Tsuyoshi

  Department of Life Science, International Christian University (ICU) Gene Research Center, University of Tsukuba

Bibliographic Information

Other Title

• EMS mutagenesis and characterization of Brassica rapa mutants

Abstract

Brassica rapa is an important model crop in the genus Brassica, which includes various important vegetable crops such as Chinese cabbage and turnip, and is closely related to Arabidopsis thaliana (Arabidopsis). B. rapa var. trilocularis (yellow sarson), a rapid-cycling population of B. rapa, is commonly used for genetic research and is expected to bridge the gap between the model plant Arabidopsis and Brassica crops. In this study, 940 M₂ mutagenized lines of yellow sarson were produced using ethylmethanesulfonate (EMS) mutagenesis, and these M₂ plants were examined for alterations in visible phenotypes. In total, 293 independent M₂ lines (including 2188 M₂ plants) were investigated for phenotypic alterations, and 394 individual mutants were isolated. Subsequently, the observed mutant phenotypes were classified into 8 major categories and 18 subcategories. In addition, three mutants, namely, early flowering 1 (elf1), crane-like 1 (crl1), and rosette 2 (ros2), were selected for further phenotypic and genetic analysis. elf1 and ros2 showed early and late flowering phenotypes, respectively. Based on their phenotypes, crl1 and ros2 are likely mutations in genes for auxin signaling and gibberellic acid biosynthesis, respectively.

Journal

• Plant Biotechnology

  Plant Biotechnology 31 (2), 185-190, 2014

  Japanese Society for Plant Biotechnology

Keywords

• <i>Brassica rapa</i>
• ethylmethanesulfonate (EMS)
• flowering time
• gibberellic acid (GA)

Details

• CRID: 1390001204328720512
• NII Article ID: 130004435283
• NII Book ID: AA11250821
• DOI: 10.5511/plantbiotechnology.14.0109b
• ISSN: 13476114
• ISSN: 13424580
• NDL BIB ID: 025551132
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025551132
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed