Role of Fatty Acid Synthase in the Development of <i>Dictyostelium discoideum</i>

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    • Sato Yukie G
    • Graduate School of Science and Technology, Sophia University
    • Morita Naoki
    • Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
    • Saito Tamao
    • Department of Materials and Life Sciences, Sophia University


Fatty acids are fundamental cellular components, and provide essential building blocks for membrane biosynthesis. Although the use of gene knockout mutants is a robust method for examining the function of specific cellular metabolic networks, fatty acid synthase knockout mutants are extremely difficult to isolate. In the <i>Dictyostelium discoideum</i> genome, we found two putative fatty acid synthase genes, and we created a knockout mutant for one of them to examine the physiological consequences. In this study, we found that a continuous fatty acid supply was necessary for normal development, and the fatty acid synthase knockout mutant showed severe developmental delay. This developmental defect was corrected in chimeras composed of wild type cells and knockout mutant cells (3:7, respectively). The knockout mutant also showed aberrant expression of fatty acid biosynthesis genes. These results showed that <i>D. discoideum</i> needs correct fatty acid synthesis for normal development.


  • Journal of Oleo Science

    Journal of Oleo Science 63(3), 281-289, 2014

    Japan Oil Chemists' Society


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