Embryo Development and the Mechanisms behind the Improvements in In Vitro Dynamic Culture Systems

DOI
  • Matsuura Koji
    Research Core for Interdisciplinary Sciences, Okayama University
  • Naruse Keiji
    Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

Bibliographic Information

Other Title
  • Dynamic Culture Systemによる受精卵発育促進とその機構解明
  • Embryo Development and the Mechanisms behind the Improvements in<i>In Vitro</i>Dynamic Culture Systems

Abstract

Mammalian embryo culture is influenced by autocrine and paracrine effects and the diffusion of harmful byproducts. The physiological development of the embryo occurs in the Fallopian tube under dynamic conditions. To obtain high quality embryos in in vitro culture, dynamic culture systems (DCSs) have been developed which mimic the dynamic physiological conditions. In this review, embryo culture using DCSs in vitro and their culture results are discussed. From the culture results of DCSs that move the medium and embryos, we deduced that an increase in the diffusion rate is one of the reasons for an improvement in embryo development. Diffusion around the embryos could be investigated by monitoring pH changes in the medium during embryo culture. Application of moderate mechanical stimuli to embryos using DCSs would induce improvement in embryo development. To understand mechanosensing of mammalian embryos, mechanical stimuli and intracellular calcium concentrations in the embryos should be quantitatively evaluated. Finally, strategies to apply these insights and technologies to human embryo development are briefly discussed.<br>

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Details 詳細情報について

  • CRID
    1390282679316493568
  • NII Article ID
    130004436816
  • DOI
    10.1274/jmor.28.174
  • ISSN
    13475878
    13417738
  • Text Lang
    en
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
    • KAKEN
  • Abstract License Flag
    Disallowed

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