Competition between Polysaccharide/Polynucleotide Complexation vs. Polynucleotide Hybridization; Salt Concentration Dependence of the Reaction Direction
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- Karinaga Ryouji
- Department of Chemical Process & Environments, The University of Kitakyushu, Japan
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- Koumoto Kazuya
- Department of Chemical Process & Environments, The University of Kitakyushu, Japan
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- Minari Jusaku
- Department of Chemical Process & Environments, The University of Kitakyushu, Japan
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- Mizu Masami
- Department of Chemical Process & Environments, The University of Kitakyushu, Japan
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- Anada Takahisa
- Department of Chemical Process & Environments, The University of Kitakyushu, Japan
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- Sakurai Kazuo
- Department of Chemical Process & Environments, The University of Kitakyushu, Japan
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- Shinkai Seiji
- Faculty of Engineering, Department of Chemistry & Biochemistry, Graduate School of Engineering, Kyushu University, Japan
Abstract
Schizophyllan is a natural β-(1→3)-D-glucan existing as a triple helix in water and as a single chain in dimethylsulfoxide (DMSO), respectively. As we already reported, when a homo-polynucleotide [ex. poly(A) or poly(dA)] was added to the schizophyllan/DMSO solution and subsequently DMSO was exchanged for water, the single chain of schizophyllan formed a complex with the polynucleotide. In this paper, using an adenine-thymine (or uracil) double strand as a model system, we explored whether s-SPG can unzip the polynucleotide duplexes, examining the competition between the schizophyllan/polynucleotide complexation and the polynucleotide hybridization with circular dichroism and fluorescence spectroscopy at various NaCl concentrations. The unzipping reaction happened for the hetero-duplexes (i.e., RNA-DNA duplexes) at low salt concentrations, on the other hand, it was not observed for the homo-duplexes at all the salt concentrations. When we compared dissociation temperatures, the unzipping reaction of the homo-duplexes was expected at low salt concentrations. This discrepancy between the experimental results and the expectation, as well as the different behavior between the hetero- and homo- duplexes, can be ascribed to the difference in the activation energy of intermediate states. [DOI: 10.1380/ejssnt.2005.38]
Journal
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- e-Journal of Surface Science and Nanotechnology
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e-Journal of Surface Science and Nanotechnology 3 38-45, 2005
The Japan Society of Vacuum and Surface Science
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Keywords
Details 詳細情報について
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- CRID
- 1390282680163960192
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- NII Article ID
- 130004438927
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- ISSN
- 13480391
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed