The Single GUV Method for Probing Biomembrane Structure and Function

• Yamazaki Masahito

  Department of Physics, Faculty of Science, Shizuoka University, Japan Materials Science, Graduate School of Science and Engineering, Shizuoka University, Japan

• Tamba Yukihiro

  Materials Science, Graduate School of Science and Engineering, Shizuoka University, Japan

Abstract

Giant liposomes or giant unilamellar vesicles (GUVs) of lipid membranes, with diameter greater than 10 μm have a great advantage over smaller liposomes, e.g., large or small unilamellar vesicles, LUVs or SUVs in the investigation of biomembranes. Studies of single GUVs (the 'single GUV method') yield especially useful information on their structure and physical properties as a function of time and spatial coordinates. Here we show three examples of studies using the single GUV method: analysis of shape changes of GUVs; membrane fusion and vesicle fission; and use of a GUV as a microscopic container. [DOI: 10.1380/ejssnt.2005.218]

Journal

• e-Journal of Surface Science and Nanotechnology

  e-Journal of Surface Science and Nanotechnology 3 218-227, 2005

  The Japan Society of Vacuum and Surface Science

Keywords

• Biophysics
• Bioimaging
• Self-assembly
• Thermodynamic properties
• Lanthanides
• Membrane Interface
• Giant Unilamellar Vesicle (GUV)
• Membrane Fusion and Fission

Details

• CRID: 1390282680163867648
• NII Article ID: 130004438947
• DOI: 10.1380/ejssnt.2005.218
• ISSN: 13480391
• Web Site: http://hdl.handle.net/10297/9126
• Web Site: http://www.jstage.jst.go.jp/article/ejssnt/3/0/3_0_218/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed