Single Molecule Tracking of Cholera-Toxin Subunit B on GM1-ganlioside Containing Lipid Bilayer

• Nabika Hideki

  Division of Chemistry, Graduate School of Science, Hokkaido University, Japan

• Motegi Toshinori

  Division of Chemistry, Graduate School of Science, Hokkaido University, Japan

• Murakoshi Kei

  Division of Chemistry, Graduate School of Science, Hokkaido University, Japan

抄録

Diffusivity of Cholera toxin subunit B (CTX) bind to GM1 molecules in 1,2-Dioleoyl-sn-Glycero-3-Phosphocholine (DOPC) bilayer was observed by total internal reflection microscope (TIRFM). Numerical analysis based on mean square displacement revealed the presence of higher (D = 0.4 μm²/s) and lower (D < 0.1 μm²/s) diffusive fractions, both of which were an ordered of slower compared to dye-labeled lipid (D = 3.5 μm²/s). The observed difference in CTX-GM1 diffusivity reflects the characteristics of the multivalent CTX-GM1 binding properties. [DOI: 10.1380/ejssnt.2009.74]

収録刊行物

• e-Journal of Surface Science and Nanotechnology

  e-Journal of Surface Science and Nanotechnology 7 74-77, 2009

  公益社団法人 日本表面真空学会

キーワード

• Single molecule observation
• Total internal reflection fluorescence microscopy
• Lipid bilayer
• Cholera toxin

詳細情報

• CRID: 1390001205186872192
• NII論文ID: 130004439119
• DOI: 10.1380/ejssnt.2009.74
• ISSN: 13480391
• Web Site: http://www.jstage.jst.go.jp/article/ejssnt/7/0/7_0_74/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可