Homogeneous DNA-detection Based on the Non-enzymatic Reactions Promoted by Target DNA

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Author(s)

    • Ihara Toshihiro IHARA Toshihiro
    • Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University|PRESTO, Japan Science and Technology Agency
    • Mukae Motoko MUKAE Motoko
    • Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University

Abstract

Much effort has focused on methods for detecting various genetic differences in individuals, including single nucleotide polymorphisms (SNPs). SNP can be characterized as a substitution, insertion, or deletion at a single base position on a DNA strand. There is expected to be on average one SNP for every 1000 bases of the human genome, and some variations located in genes are suspected to alter both the protein structure and the expression level. Therefore, highly sensitive techniques with a simple procedure would be desirable for a high-throughput screening of millions of SNPs widely dispersed throughout the human genome. In this short review, we consider recently reported unique techniques for genotyping in a homogeneous solution, and organize them in terms of the chemical and physical processes accelerated on DNA.

Journal

  • Analytical Sciences

    Analytical Sciences 23(6), 625-629, 2007

    The Japan Society for Analytical Chemistry

Cited by:  1

Codes

  • NII Article ID (NAID)
    130004441457
  • NII NACSIS-CAT ID (NCID)
    AA10500785
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0910-6340
  • NDL Article ID
    8755923
  • NDL Source Classification
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL Call No.
    Z54-F482
  • Data Source
    CJPref  NDL  J-STAGE 
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