Direct Observation of Multiple and Stochastic Transition States by a Feedback-controlled Single-molecule Force Measurement

• FUKAGAWA Akihiro

  Structural Biology Center, National Institute of Genetics Research Center for Allergy and Immunology, RIKEN

• HIROSHIMA MichioA

  Structural Biology Center, National Institute of Genetics Research Center for Allergy and Immunology, RIKEN

• SAKANE Isao

  Structural Biology Center, National Institute of Genetics

• TOKUNAGA Makio

  Structural Biology Center, National Institute of Genetics Research Center for Allergy and Immunology, RIKEN Department of Genetics, School of Life Science, The Graduate University for Advanced Studies (Sokendai) Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology

Abstract

To overcome the ensemble-averaging barrier, single-molecule experiments have been performed, but energy landscapes comprising multiple intermediates have not yet been defined. We performed mechanical unfolding of staphylococcal nuclease using intermolecular force microscopy, modified AFM with high resolution and feedback control of the positioning. The force dropped vertically just after its peak, and multiple transition states were detected as force peaks. The multiple and stochastic intermediates found in the present study provide new important information on protein folding.

Journal

• Analytical Sciences

  Analytical Sciences 25 (1), 5-7, 2009

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390001204255555328
• NII Article ID: 130004441745
• NII Article ID: 10025209071
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.25.5
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 9759321
• Web Site: http://id.ndl.go.jp/bib/9759321
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9759321
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.25.5.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.25.5/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/25/1/25_1_5/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed