Rapid Assay of Trace Ceruloplasmin Using an Immunonanogold Resonance Scattering Spectral Probe

• JIANG Zhiliang

  School of Environment and Resource, Guangxi Normal University Department of Material and Chemical Engineering, Guilin University of Technology

• WEI Lili

  School of Environment and Resource, Guangxi Normal University

• LIANG Aihui

  Department of Material and Chemical Engineering, Guilin University of Technology

抄録

Nanogold particles of size 15 nm were used to label goat anti-human ceruloplasmin (GCP) to obtain an immunonanogold probe (AuGCP) for ceruloplasmin (CP). In a pH 7.8 citric acid-Na₂HPO₄ buffer solution and in the presence of polyethylene glycol (PEG), an immunoreaction between AuGCP and CP took place, and the released nanogold particles aggregated to bigger clusters, which caused the resonance scattering (RS) intensity at 552 nm (I_552nm) to be enhanced greatly. The enhanced intensity ΔI was proportional to the CP concentration (C_CP) in the range from 0.0030 to 1.26 µg/mL, with a regress equation of ΔI = 101.0C_CP + 1.8, a correlation coefficient of 0.9970, and a detection limit of 1.1 ng/mL CP. This simple and sensitive RS immunoassay was applied to the determination of CP in human plasma, with satisfactory results.

収録刊行物

• Analytical Sciences

  Analytical Sciences 25 (4), 463-467, 2009

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390001204256037760
• NII論文ID: 130004441842
• NII論文ID: 10025211409
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.25.463
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 10201470
• Web Site: http://id.ndl.go.jp/bib/10201470
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10201470
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.25.463.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.25.463/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/25/4/25_4_463/_pdf
• 本文言語コード: en
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