Sequential Injection Immunoassay for Environmental Measurements
-
- SOH Nobuaki
- Faculty of Agriculture, Saga University
-
- TANAKA Mayumi
- Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
-
- HIRAKAWA Koji
- Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
-
- ZHANG RuiQi
- Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
-
- NAKAJIMA Hizuru
- Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
-
- NAKANO Koji
- Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
-
- IMATO Toshihiko
- Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
Abstract
Sequential injection immunoassay systems for environmental measurements based on the selective immunoreaction between antigen and antibody were described. A sequential injection analysis (SIA) technique is suitable to be applied for the procedure of enzyme-linked immunosorbent assay (ELISA), because the washing and the addition of reagent solutions can be automated by using a computer-controlled syringe pump and switching valve. We selected vitellogenin (Vg), which is a biomarker for evaluating environmental risk caused by endocrine-disrupting chemicals in the hydrosphere, and linear alkylbenzene sulfonates (LAS) and alkylphenol polyethoxylates (APEO), which are versatile surfactants, as target analytes in the flow immunoassay systems. For Vg monitoring, SIA systems based on spectrophotometric, chemiluminescence, and electrochemical determinations were constructed. On the other hand, chemiluminescence determination was applied to the detection of LAS and APEO. For APEO, an SIA system combined with surface plasmon resonance (SPR) sensor was also developed. These new sequential injection immunoassay systems are expected to be useful systems for environmental analysis.
Journal
-
- Analytical Sciences
-
Analytical Sciences 27 (11), 1069-, 2011
The Japan Society for Analytical Chemistry