Evaluation of a Method to Quantify Quercetin Aglycone in Onion (Allium cepa) by Single- and Multi-laboratory Validation Studies

• WATANABE Jun

  National Food Research Institute, National Agriculture and Food Research Organization

• TAKEBAYASHI Jun

  National Institute of Health and Nutrition

• Takano-ISHIKAWA Yuko

  National Food Research Institute, National Agriculture and Food Research Organization

• YASUI Akemi

  National Food Research Institute, National Agriculture and Food Research Organization

書誌事項

タイトル別名

• Evaluation of a Method to Quantify Quercetin Aglycone in Onion (<i>Allium cepa</i>) by Single- and Multi-laboratory Validation Studies

抄録

Official Methods of Analysis of AOAC International (OMA) 2006.07 was originally designed for quantifying flavonol aglycones in ginkgo dietary supplements. To determine whether the method is applicable to the quantification of flavonol aglycones in lyophilized onion samples, single- and multi-laboratory validation studies were performed. Triplicated measurements on 3 different days revealed that the mean quercetin content was 3.48 g/kg dry weight, and the relative repeatability standard deviation (RSD_r) and the relative intermediate standard deviation (RSD_int) were 0.8 and 1.8%, respectively. The recovery of quercetin-3-O-glucoside spiked at 3 different amounts (1.56, 3.12, and 6.24 g/kg dry weight of onion) ranged from 98.42 to 100.31%, and the RSD_r and RSD_int ranged from 2.2 to 5.9%, and from 3.4 to 5.2%, respectively. A multi-laboratory validation study showed that the mean quercetin contents were 2.80 and 6.61 g/kg dry weight, and that satisfactory inter-laboratory precision (RSD_r and RSD_R ranged from 0.41 to 0.92%, and from 6.73 to 7.62%, respectively); all HorRat values were less than 2. These results indicate that OMA 2006.07 is applicable to the determination of the quercetin content of lyophilized onion samples.

収録刊行物

• Analytical Sciences

  Analytical Sciences 28 (12), 1179-1182, 2012

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390001204259009280
• NII論文ID: 130004442104
• NII論文ID: 10031136988
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.28.1179
• COI: 1:STN:280:DC%2BC3s3htlWluw%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 024153577
• PubMed: 23232238
• Web Site: http://id.ndl.go.jp/bib/024153577
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024153577
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.28.1179.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.28.1179/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/28/12/28_1179/_pdf
• 本文言語コード: en
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