Direct Drug Metabolism Monitoring in a Live Single Hepatic Cell by Video Mass Spectrometry

• DATE Sachiko

  Quantitative Biology Center (QBiC), RIKEN

• MIZUNO Hajime

  Graduate School of Biomedical Sciences, Hiroshima University

• TSUYAMA Naohiro

  Graduate School of Biomedical Sciences, Hiroshima University

• HARADA Takanori

  Graduate School of Biomedical Sciences, Hiroshima University

• MASUJIMA Tsutomu

  Quantitative Biology Center (QBiC), RIKEN Graduate School of Biomedical Sciences, Hiroshima University

抄録

The metabolism of anti-breast cancer drug, tamoxifen, in a single human hepatocellular carcinoma cell, HepG2, was directly monitored by a video-mass spectroscope. The cytoplasm, a vacuole or nucleus of the cell was directly sucked by a nano-spray tip under a video-microscope, and then was introduced into a mass spectrometer. Unchanged drug molecules were found in cytoplasm and a vacuole, but the metabolites were only found in the cytoplasm. This direct detection of drug metabolites in a live single cell is useful for speedy drug metabolism monitoring.

収録刊行物

• Analytical Sciences

  Analytical Sciences 28 (3), 201-201, 2012

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390001204259197184
• NII論文ID: 130004442114
• NII論文ID: 10030446912
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.28.201
• COI: 1:STN:280:DC%2BC38vpvVKhtg%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 023495698
• PubMed: 22451357
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023495698
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.28.201.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.28.201/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/28/3/28_3_201/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012272980
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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