Direct Drug Metabolism Monitoring in a Live Single Hepatic Cell by Video Mass Spectrometry

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Author(s)

    • MIZUNO Hajime
    • Graduate School of Biomedical Sciences, Hiroshima University
    • MASUJIMA Tsutomu
    • Quantitative Biology Center (QBiC), RIKEN|Graduate School of Biomedical Sciences, Hiroshima University

Abstract

The metabolism of anti-breast cancer drug, tamoxifen, in a single human hepatocellular carcinoma cell, HepG2, was directly monitored by a video-mass spectroscope. The cytoplasm, a vacuole or nucleus of the cell was directly sucked by a nano-spray tip under a video-microscope, and then was introduced into a mass spectrometer. Unchanged drug molecules were found in cytoplasm and a vacuole, but the metabolites were only found in the cytoplasm. This direct detection of drug metabolites in a live single cell is useful for speedy drug metabolism monitoring.

Journal

  • Analytical Sciences

    Analytical Sciences 28(3), 201-201, 2012

    The Japan Society for Analytical Chemistry

Codes

  • NII Article ID (NAID)
    130004442114
  • Text Lang
    ENG
  • ISSN
    0910-6340
  • Data Source
    J-STAGE 
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