Quantification of Antioxidants by Using Chlorpromazine Hydrochloride: Application of the Method to Food and Medicinal Plant Samples

• NAGARAJA Padmarajaiah

  Department of Studies in Chemistry, University of Mysore

• ARADHANA Narayanan

  Department of Studies in Chemistry, University of Mysore

• SUMA Aandamurthy

  Department of Studies in Chemistry, University of Mysore

• SHIVAKUMAR Anantharaman

  Department of Studies in Chemistry, University of Mysore

• CHAMARAJA Nelligere Arkeshwaraiah

  Department of Studies in Chemistry, University of Mysore

Abstract

Chlorpromazine hydrochloride (CPH) (3-(2-chloro-phenothiazine-10-yl)-propyl] dimethylamine hydrochloride) has been the subject of a large number of studies employing a broad spectrum of oxidants, and chosen to examine the course of electron transfer reactions. We report on a method to determine the antioxidant activity of some food and medicinal plants using the oxidation of CPH by chromium(VI) to form a stable CPH radical in the 1:1 orthophosphoric acid–ethyl alcohol (OPA-EtOH) medium. The pink color of the control solution was measured at λ_max of 530 nm. Nine standard antioxidants have been studied by this method, along with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The EC₅₀, TEC₅₀, antioxidant efficacy and the stoichiometric values for antioxidants have been evaluated. The radical scavenging activity expressed as EC₅₀ ranged from 9.2 μg/mL in Camellia sinensis to 448.18 μg/mL in Cuminum cyminum. The application of a simple and versatile antioxidant capacity assay for dietary polyphenols and medicinal plant extracts, which are commonly used in Ayurveda opens its relevance in the field of antioxidant analysis.

Journal

• Analytical Sciences

  Analytical Sciences 30 (2), 251-256, 2014

  The Japan Society for Analytical Chemistry

Keywords

• Antioxidants
• chlorpromazine hydrochloride
• chromium(VI)
• spectrophotometry
• free radical

Details

• CRID: 1390282679238196480
• NII Article ID: 130004442261
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.30.251
• COI: 1:STN:280:DC%2BC2cvkslaisg%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 025287282
• PubMed: 24521912
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025287282
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.30.251.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.30.251/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/30/2/30_251/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015061523
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed