Split Luciferase Complementation for Analysis of Intracellular Signaling

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Author(s)

Abstract

Bioluminescent proteins such as luciferases are unique analytical tools with high sensitivity and wide dynamic ranges. However, applicability of the proteins has remained limited to reporter gene analysis. Split luciferase complementation technique is a new powerful strategy to detect protein–protein interactions and their related intracellular signaling in living cells. This review presents a summary of recent topics for the analysis of biological events using split luciferase complementation techniques. Different applications such as G-protein-coupled receptor (GPCR) screening, monitoring phosphorylations, protein–protein interactions, and pH differences in living tissues are described.

Journal

  • Analytical Sciences

    Analytical Sciences 30(5), 539-544, 2014

    The Japan Society for Analytical Chemistry

Codes

  • NII Article ID (NAID)
    130004442285
  • NII NACSIS-CAT ID (NCID)
    AA10500785
  • Text Lang
    ENG
  • ISSN
    0910-6340
  • NDL Article ID
    025440653
  • NDL Call No.
    Z54-F482
  • Data Source
    NDL  J-STAGE 
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