Measurement of <i>TFF3</i> mRNA in aspirates from thyroid nodules using mesh filtration: The first clinical trial in 130 cases

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  • Yamada Hiroya
    Department of Laboratory Medicine, Osaka University Graduate School of Medicine, Suita 565-0871, Japan Division of Health Sciences, Osaka University Graduate School of Medicine, Suita 565-0871, Japan Department of Laboratory Medicine, Osaka University Graduate School of Medicine, Suita 565-0871, Japan Division of Health Sciences, Osaka University Graduate School of Medicine, Suita 565-0871, Japan
  • Takano Toru
    Department of Laboratory Medicine, Osaka University Graduate School of Medicine, Suita 565-0871, Japan Department of Laboratory Medicine, Osaka University Graduate School of Medicine, Suita 565-0871, Japan
  • Kihara Minoru
    Kuma Hospital, Kobe 650-0011, Japan Kuma Hospital, Kobe 650-0011, Japan
  • Hirokawa Mitsuyoshi
    Kuma Hospital, Kobe 650-0011, Japan Kuma Hospital, Kobe 650-0011, Japan
  • Yoshida Hiroshi
    Kuma Hospital, Kobe 650-0011, Japan Kuma Hospital, Kobe 650-0011, Japan
  • Watanabe Mikio
    Division of Health Sciences, Osaka University Graduate School of Medicine, Suita 565-0871, Japan Division of Health Sciences, Osaka University Graduate School of Medicine, Suita 565-0871, Japan
  • Iwatani Yoshinori
    Division of Health Sciences, Osaka University Graduate School of Medicine, Suita 565-0871, Japan Division of Health Sciences, Osaka University Graduate School of Medicine, Suita 565-0871, Japan
  • Hidaka Yoh
    Department of Laboratory Medicine, Osaka University Graduate School of Medicine, Suita 565-0871, Japan Department of Laboratory Medicine, Osaka University Graduate School of Medicine, Suita 565-0871, Japan
  • Miyauchi Akira
    Kuma Hospital, Kobe 650-0011, Japan Kuma Hospital, Kobe 650-0011, Japan

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抄録

Measurement of gene expression levels in thyroid tumor cells in aspirates was difficult because it is interfered with peripheral blood cells or infiltrating lymphocytes. In this study, we established a novel method to separate thyroid tumor cells from blood cells efficiently with mesh filtration. The expression level of trefoil factor 3 (TFF3) mRNA was estimated using LGALS3 mRNA as an internal control (T/G ratio) in 148 preoperative thyroid aspirates. Intra-assay coefficients of variation (CV) of T/G ratio for high, moderate, and low samples were 6.5%, 2.5%, and 9.7%, respectively, and inter-assay CV for high, moderate, and low samples were 27.7%, 21.9%, and 38.2%, respectively. Nondiagnostic samples in terms of T/G ratio and cytology were 12.2% and 16.9%, respectively. We observed no interference with the data by contaminating blood cells. Among these patients, 12 patients received more than two repeated aspirations. We did not observe a marked day-to-day variation except in two cases. All 13 preoperative aspirates diagnosed as malignant by cytology showed an extremely low T/G ratio, whereas 93 aspirates diagnosed as benign by cytology showed extremely varied T/G ratios and 21.5% of them showed a T/G ratio below the cut-off value. Eleven cases underwent surgery. All nodules showing a low T/G ratio were diagnosed as papillary carcinoma by pathological diagnosis. However, one nodule diagnosed as follicular adenoma after surgery showed a high T/G ratio. Our present method may be a promising preoperative test for measuring mRNAs in thyroid aspirates.

収録刊行物

  • Endocrine Journal

    Endocrine Journal 59 (7), 621-630, 2012

    一般社団法人 日本内分泌学会

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