Anti-Atherosclerotic Effects of Dihomo-.GAMMA.-Linolenic Acid in ApoE-Deficient Mice
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- Takai Shinji
- Department of Pharmacology, Osaka Medical College
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- Jin Denan
- Department of Pharmacology, Osaka Medical College
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- Kawashima Hiroshi
- Institute for Health Care Science, Suntory Ltd.
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- Kimura Maki
- Department of Clinical Pharmacy and Clinical Pharmacokinetics, Osaka University of Pharmaceutical Sciences
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- Shiraishi-Tateishi Akiko
- Institute for Health Care Science, Suntory Ltd.
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- Tanaka Takao
- Institute for Health Care Science, Suntory Ltd.
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- Kakutani Saki
- Institute for Health Care Science, Suntory Ltd.
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- Tanaka Kazuhiko
- Department of Clinical Pharmacy and Clinical Pharmacokinetics, Osaka University of Pharmaceutical Sciences
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- Kiso Yoshinobu
- Institute for Health Care Science, Suntory Ltd.
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- Miyazaki Mizuo
- Department of Pharmacology, Osaka Medical College
抄録
Aim: Dihomo-γ-linolenic acid (DGLA) is an n-6 polyunsaturated fatty acid that is mainly metabolized to an anti-inflammatory eicosanoid, prostaglandin (PG) E1, via the cyclooxygenase (COX) pathway. We evaluated the effect of DGLA on atherosclerosis in apoE-deficient mice and studied the mechanism of the anti-atherosclerotic effect.<BR>Methods: ApoE-deficient mice were fed a normal diet supplemented with 0.5% DGLA or vehicle for 6 months. ApoE-deficient mice were also fed a high-cholesterol diet supplemented with 0.5% DGLA or vehicle for 1 month. To clarify the influence of a COX inhibitor, naproxen, on the anti-atherosclerotic effect of DGLA, age-matched apoE-deficient mice fed a high-cholesterol diet supplemented with 0.5% DGLA were given oral naproxen for 1 month.<BR>Results: In normal diet-fed mice, acetylcholine-induced vascular relaxation was significantly greater in the DGLA group than in the vehicle group. NADPH oxidase subunits, p22phox and gp91phox, intercellular adhesion molecule-1, and vascular cellular adhesion molecule-1 were significantly lower in the DGLA group than in the vehicle group, and DGLA significantly prevented atherosclerosis. In high-cholesterol diet-fed mice, DGLA also significantly prevented atherosclerosis, but the anti-atherosclerotic effect was attenuated by naproxen.<BR>Conclusion: DGLA may have an anti-atherosclerotic effect in apoE-deficient mice via PGE1 formation.
収録刊行物
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- Ｊｏｕｒｎａｌ ｏｆ Ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ ａｎｄ Ｔｈｒｏｍｂｏｓｉｓ
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Ｊｏｕｒｎａｌ ｏｆ Ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ ａｎｄ Ｔｈｒｏｍｂｏｓｉｓ 16 (4), 480-489, 2009
一般社団法人 日本動脈硬化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679408642432
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- NII論文ID
- 130004444309
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- ISSN
- 18803873
- 13403478
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
