Imaging of structural changes in endothelial cells and thrombus formation at the site of FeCl(3)-induced injuries in mice cremasteric arteries

• Kawamura Yota

  Department of Medicine (Cardiology), Center for Metabolic Disease Research, Bioresearch Center, Tokai University School of Medicine.

• Takahari Yoko

  Teaching and Research Support Center, Tokai University School of Medicine.

• Tamura Noriko

  Department of Medicine (Cardiology), Center for Metabolic Disease Research, Bioresearch Center, Tokai University School of Medicine.

• Eguchi Yu

  Department of Physiology, Tokai University School of Medicine.

• Urano Tetsuya

  Department of Medicine (Cardiology), Center for Metabolic Disease Research, Bioresearch Center, Tokai University School of Medicine.

• Ishida Hideyuki

  Department of Physiology, Tokai University School of Medicine.

• Goto Shinya

  Department of Medicine (Cardiology), Center for Metabolic Disease Research, Bioresearch Center, Tokai University School of Medicine.

書誌事項

タイトル別名

• Imaging of Structural Changes in Endothelial Cells and Thrombus Formation at the Site of FeCl₃-Induced Injuries in Mice Cremasteric Arteries

抄録

Aim: We investigated thrombus formation at the site of functional injury to endothelial cells by FeCl₃.<BR>Methods: After preparation of cremasteric arteries of mice, controlled endothelial injury was induced by application of FeCl₃. Endothelial cells were rendered fluorescent by addition of FITC (fluorescein isothiocyanate)-labeled isolectin B4. Circulating platelets and leukocytes were made fluorescent by rhodamine 6G. Three-dimensional (3D) growth of thrombi was visualized in real time. Effects of aspirin and clopidogrel pre-treatments on the growth of thrombi were investigated in vivo as well as in an ex vivo flow chamber system.<BR>Results: Endothelial cells were tightly bound to each other to protect local thrombus formation. Platelets started to adhere to endothelial cells when FeCl₃ was applied. Three-dimensional growth of thrombi, which takes 10.6±7.5 minutes for complete occlusion in control, can be visualized with our imaging system. Aspirin pre-treatment at the dose tested did not influence either endothelial injury or platelet thrombus growth, while clopidogrel pretreatment significantly inhibited 3D growth and prolonged occlusion time up to 64.6±25.3 minutes (100 mg/kg). A similar inhibiting effect of clopidogrel was reproduced in ex vivo flow chamber experiments.<BR>Conclusions: We have developed an in vivo system to detect thrombus formation after functional damage to the endothelium.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ　ａｎｄ　Ｔｈｒｏｍｂｏｓｉｓ

  Ｊｏｕｒｎａｌ　ｏｆ　Ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ　ａｎｄ　Ｔｈｒｏｍｂｏｓｉｓ 16 (6), 807-814, 2009

  一般社団法人 日本動脈硬化学会

キーワード

• Endothelial cell
• Imaging
• Platelet
• Thrombi
• Animal model
• FeCl₃

詳細情報

• CRID: 1390282679408489728
• NII論文ID: 130004444350
• DOI: 10.5551/jat.2030
• ISSN: 18803873
• ISSN: 13403478
• Web Site: https://www.jstage.jst.go.jp/article/jat/16/6/16_2030/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010153001
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可