Isolation of Gametophytic Cells and Identification of Their Cell-Specific Markers in Torenia fournieri, T. concolor and Lindernia micrantha

• Kawano Nao

  Division of Biological Science, Graduate School of Science, Nagoya University

• Susaki Daichi

  Division of Biological Science, Graduate School of Science, Nagoya University

• Sasaki Narie

  Division of Biological Science, Graduate School of Science, Nagoya University

• Higashiyama Tetsuya

  Division of Biological Science, Graduate School of Science, Nagoya University ERATO Live-Holonics Project, Japan Science and Technology Agency (JST)

• Kanaoka Masahiro M.

  Division of Biological Science, Graduate School of Science, Nagoya University

抄録

Gametophytic cells in the female embryo sac, the egg, the central and the synergid cells have unique roles in sexual reproduction in higher plants. To investigate their cell-specific features, an efficient method of distinguishing and isolating each gametophytic cell is required. By testing the activities of several cellulase enzymes, we modified our previous cell isolation method and succeeded in isolating each cell from the Torenia and Lindernia species. We also established genetic markers that show specific expression patterns in each gametophytic cell. These markers, together with the cell isolation method, will be useful for analyzing the function of gametophytic cells.

収録刊行物

• ＣＹＴＯＬＯＧＩＡ

  ＣＹＴＯＬＯＧＩＡ 76 (2), 177-184, 2011

  公益財団法人 日本メンデル協会

キーワード

• Egg cell
• Central cell
• Synergid cell
• Protoplast isolation
• Gametophytic cell-specific markers
• <i>Torenia</i>

詳細情報

• CRID: 1390001204108931072
• NII論文ID: 130004448119
• DOI: 10.1508/cytologia.76.177
• ISSN: 13487019
• ISSN: 00114545
• Web Site: http://www.jstage.jst.go.jp/article/cytologia/76/2/76_2_177/_pdf
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可