Evidence for Avt6 as a vacuolar exporter of acidic amino acids in <italic>Saccharomyces cerevisiae</italic> cells
-
- Chahomchuen Thippayarat
- Faculty of Agriculture, Ehime University
-
- Hondo Kana
- Faculty of Agriculture, Ehime University
-
- Ohsaki Mariko
- Faculty of Agriculture, Ehime University
-
- Sekito Takayuki
- Faculty of Agriculture, Ehime University
-
- Kakinuma Yoshimi
- Faculty of Agriculture, Ehime University
書誌事項
- タイトル別名
-
- Evidence for Avt6 as a vacuolar exporter of acidic amino acids in Saccharomyces cerevisiae cells
抄録
Here we examined the significance of Avt6, a vacuolar exporter of glutamate and aspartate suggested by the in vitro membrane vesicle experiment, in vacuolar compartmentalization of amino acids in Saccharomyces cerevisiae cells. Fluorescent microscopic observation of GFP-fused Avt6 revealed it to be exclusively localized to the vacuolar membrane, with the amount of Myc-tagged Avt6 significantly increased under nitrogen starvation. Glutamate uptake by cells was enhanced by deletion of the AVT6 gene, indicating indirect involvement of Avt6 in cellular glutamate accumulation. Differences in acidic amino acid content of both total and vacuolar fractions were insignificant between the parent and avt6Δ cells when cultured in nutrient-rich conditions. However, in nitrogen-starved conditions, the amount of glutamate and aspartate in the vacuolar fraction was notably increased in the avt6Δ cells. Avt6 is thus involved in vacuolar amino acid compartmentalization in S. cerevisiae cells, especially under conditions of nitrogen starvation.
収録刊行物
-
- The Journal of General and Applied Microbiology
-
The Journal of General and Applied Microbiology 55 (6), 409-417, 2009
公益財団法人 応用微生物学・分子細胞生物学研究奨励会