Single mutations introduced in the essential ribosomal proteins L3 and S10 cause a sporulation defect in <i>Bacillus subtilis</i>

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Author(s)

    • Akanuma Genki
    • Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University
    • Iizuka Yuka
    • Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University
    • Kobayashi Ako
    • Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University
    • Ishizuka Morio
    • Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University
    • Kawamura Fujio
    • Laboratory of Molecular Genetics, Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University
    • Suzuki Shota
    • Laboratory of Molecular Genetics, Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University
    • Yano Koichi
    • Laboratory of Molecular Genetics, Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University
    • Nanamiya Hideaki
    • Laboratory of Molecular Genetics, Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University
    • Natori Yousuke
    • Laboratory of Molecular Genetics, Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University
    • Namba Eri
    • Laboratory of Molecular Genetics, Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University
    • Watanabe Kazuya
    • Laboratory of Molecular Genetics, Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University
    • Tagami Kazumi
    • Laboratory of Molecular Genetics, Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University
    • Takeda Takuya
    • Laboratory of Molecular Genetics, Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University

Abstract

We introduced single mutations into the <i>rplC</i> and <i>rpsJ</i> genes, which encode the essential ribosomal proteins L3 (RplC) and S10 (RpsJ), respectively, and are located in the S10 gene cluster of the gram-positive, endospore-forming bacterium <i>Bacillus subtilis</i>, and examined whether these mutations affected their growth rate, sporulation, competence development and 70S ribosome formation. Mutant cells harboring the G52D mutation in the L3 ribosomal protein, which is located at the peptidyl transferase center of 50S, accumulated 30S subunit at 45°C, probably due to a defect in 50S formation, and exhibited a reduction in the sporulation frequency at high temperature. On the other hand, mutant cells harboring the H56R mutation in the S10 protein, which is located near the aminoacyl-tRNA site of 30S, showed severe growth defect and deficiency in spore formation, and also exhibited significant delay in competence development.

Journal

  • The Journal of General and Applied Microbiology

    The Journal of General and Applied Microbiology 59(2), 105-117, 2013

    Applied Microbiology, Molecular and Cellular Biosciences Research Foundation

Codes

  • NII Article ID (NAID)
    130004449359
  • Text Lang
    ENG
  • ISSN
    0022-1260
  • Data Source
    J-STAGE 
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