Single mutations introduced in the essential ribosomal proteins L3 and S10 cause a sporulation defect in <i>Bacillus subtilis</i>
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We introduced single mutations into the <i>rplC</i> and <i>rpsJ</i> genes, which encode the essential ribosomal proteins L3 (RplC) and S10 (RpsJ), respectively, and are located in the S10 gene cluster of the gram-positive, endospore-forming bacterium <i>Bacillus subtilis</i>, and examined whether these mutations affected their growth rate, sporulation, competence development and 70S ribosome formation. Mutant cells harboring the G52D mutation in the L3 ribosomal protein, which is located at the peptidyl transferase center of 50S, accumulated 30S subunit at 45°C, probably due to a defect in 50S formation, and exhibited a reduction in the sporulation frequency at high temperature. On the other hand, mutant cells harboring the H56R mutation in the S10 protein, which is located near the aminoacyl-tRNA site of 30S, showed severe growth defect and deficiency in spore formation, and also exhibited significant delay in competence development.
- The Journal of General and Applied Microbiology
The Journal of General and Applied Microbiology 59(2), 105-117, 2013
Applied Microbiology, Molecular and Cellular Biosciences Research Foundation