Identification of photoactivated adenylyl cyclases in Naegleria australiensis and BLUF-containing protein in Naegleria fowleri
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- Yasukawa Hiro
- Academic Group of Applied Life Sciences, Iwate University
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- Sato Aya
- Graduate School of Science and Engineering, University of Toyama
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- Kita Ayaka
- Graduate School of Science and Engineering, University of Toyama
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- Kodaira Ken-ichi
- Graduate School of Science and Engineering, University of Toyama
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- Iseki Mineo
- Graduate School of Pharmaceutical Sciences, Toho University
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- Takahashi Tetsuo
- Graduate School of Pharmaceutical Sciences, Toho University
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- Shibusawa Mami
- Graduate School of Pharmaceutical Sciences, University of Tokyo
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- Watanabe Masakatsu
- Graduate School for the Creation of New Photonics Industries
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- Yagita Kenji
- Department of Parasitology, National Institute of Infectious Diseases
書誌事項
- タイトル別名
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- Identification of photoactivated adenylyl cyclases in <i>Naegleria australiensis</i> and BLUF-containing protein in <i>Naegleria fowleri</i>
抄録
Complete genome sequencing of Naegleria gruberi has revealed that the organism encodes polypeptides similar to photoactivated adenylyl cyclases (PACs). Screening in the N. australiensis genome showed that the organism also encodes polypeptides similar to PACs. Each of the Naegleria proteins consists of a “sensors of blue-light using FAD” domain (BLUF domain) and an adenylyl cyclase domain (AC domain). PAC activity of the Naegleria proteins was assayed by comparing sensitivities of Escherichia coli cells heterologously expressing the proteins to antibiotics in a dark condition and a blue light-irradiated condition. Antibiotics used in the assays were fosfomycin and fosmidomycin. E. coli cells expressing the Naegleria proteins showed increased fosfomycin sensitivity and fosmidomycin sensitivity when incubated under blue light, indicating that the proteins functioned as PACs in the bacterial cells. Analysis of the N. fowleri genome revealed that the organism encodes a protein bearing an amino acid sequence similar to that of BLUF. A plasmid expressing a chimeric protein consisting of the BLUF-like sequence found in N. fowleri and the adenylyl cyclase domain of N. gruberi PAC was constructed to determine whether the BLUF-like sequence functioned as a sensor of blue light. E. coli cells expressing a chimeric protein showed increased fosfomycin sensitivity and fosmidomycin sensitivity when incubated under blue light. These experimental results indicated that the sequence similar to the BLUF domain found in N. fowleri functioned as a sensor of blue light.
収録刊行物
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- The Journal of General and Applied Microbiology
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The Journal of General and Applied Microbiology 59 (5), 361-369, 2013
公益財団法人 応用微生物学・分子細胞生物学研究奨励会
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詳細情報
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- CRID
- 1390282679123820160
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- NII論文ID
- 130004449379
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- COI
- 1:CAS:528:DC%2BC3sXhslOkt7jJ
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- ISSN
- 13498037
- 00221260
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- PubMed
- 24201148
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可