Isolation of PsPINs and PsAUX1 cDNAs Encoding Putative Auxin Efflux and Influx Carriers and/or Facilitators, Respectively, from Etiolated Epicotyls of an Agravitropic Pea (Pisum sativum L.) Mutant, ageotropum

• Ueda Junichi

  Graduate School of Science, Osaka Prefecture University

• Tada Tomohiro

  Graduate School of Science, Osaka Prefecture University

• Hoshino Tomoki

  Graduate School of Science, Osaka Prefecture University

• Miyamoto Kensuke

  Faculty of Liberal Arts and Sciences, Osaka Prefecture University

• Uheda Eiji

  Graduate School of Science, Osaka Prefecture University

• Oka Mariko

  Faculty of Agriculture, Tottori University

書誌事項

タイトル別名

• Isolation of <i>PsPINs</i> and <i>PsAUX1</i> cDNAs Encoding Putative Auxin Efflux and Influx Carriers and/or Facilitators, Respectively, from Etiolated Epicotyls of an Agravitropic Pea (<i>Pisum sativum</i> L.) Mutant, <i>ageotropum</i>

抄録

cDNAs containing the open reading frames of a putative auxin influx carrier and/or facilitator, PsAUX1 (Accession No. AB488680), and putative auxin efflux carriers and/or facilitators, PsPIN1, PsPIN2 and PsPIN3 (Accession No. AB488679, AB488681 and AB488678, respectively) from etiolated epicotyls of an agravitropic pea mutant, ageotropum, have been isolated. In PsPIN1 and PsPIN2, a few deduced amino acids were different between ageotropum and Alaska showing a normal gravitropic response. No insertion and deletion were observed in PsPIN1 and PsPIN2 of ageotropum compared with those of Alaska. However, PsAUX1 of ageotropum had additional amino acids at the C-terminus compared to that of Alaska. Phylogenetic analyses based on deduced amino acid sequences revealed that PsPIN1 of ageotropum and Alaska belonged to the same clade as MtPIN4, while PsPIN2 of ageotropum and Alaska belonged to a subclade including MtPIN3, BjPIN2, BjPIN3, SlPIN3 and SlPIN4. PsAUX1 of ageotropum with additional amino acids at C-terminus belonged to the same subclade as MiAUX1 and PtAUX1. PsPIN1, PsPIN2, PsPIN3 mRNAs in 3.5-day-old etiolated ageotropum pea seedlings were significantly expressed in the proximal and the distal sides of the first internode of epicotyls to cotyledon. Judging from the results of the structure and the expression of genes related to auxin polar transport, an abnormal gravitropic response of etiolated ageotropum pea seedlings might be related to post transcriptional regulation and translocation of PsAUX1, PsPIN1, PsPIN2 and PsPIN3 as well as the perturbation of auxin transport due to additional amino acids at C-terminus of PsAUX1.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ　ｉｎ　Ｓｐａｃｅ

  Ｂｉｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ　ｉｎ　Ｓｐａｃｅ 26 (0), 32-41, 2012

  日本宇宙生物科学会

キーワード

• <i>ageotropum</i>
• automorphosis-like growth
• auxin efflux carrier and/or facilitator
• auxin influx carrier and/or facilitator
• auxin polar transport
• pea
• <i>Pisum sativum</i>
• <i>PsAUX1</i>
• <i>PsPIN1</i>
• <i>PsPIN2</i>
• <i>PsPIN3</i>

詳細情報

• CRID: 1390282679410213632
• NII論文ID: 130004450624
• DOI: 10.2187/bss.26.32
• ISSN: 1349967X
• ISSN: 09149201
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可