Genome-scale DNA methylation analysis in GI cancers

• Watanabe Yoshiyuki

  Division of Gastroenterology and Hepatology, Department of Internal Medicine, St. Marianna University School of Medicine Department of Internal Medicine, Kawasaki Rinko General Hospital Leukemia Department, University of Texas, MD Anderson Cancer Center

• Yoshida Yoshihito

  Division of Gastroenterology and Hepatology, Department of Internal Medicine, St. Marianna University School of Medicine Department of Internal Medicine, Kawasaki Rinko General Hospital

• Oikawa Ritsuko

  Division of Gastroenterology and Hepatology, Department of Internal Medicine, St. Marianna University School of Medicine

• Hara Masaki

  Division of Gastroenterology and Hepatology, Department of Internal Medicine, St. Marianna University School of Medicine

• Kiyokawa Hiroshi

  Division of Gastroenterology and Hepatology, Department of Internal Medicine, St. Marianna University School of Medicine

• Sato Yoshinori

  Division of Gastroenterology and Hepatology, Department of Internal Medicine, St. Marianna University School of Medicine

• Hirai Sachiko

  Department of Gastroenterology and Hepatology, Institute of Clinical Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba

• Suzuki Hideo

  Department of Gastroenterology and Hepatology, Institute of Clinical Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba

• Tsuda Takashi

  Department of Oncology, St. Marianna University School of Medicine

• Inaba Hiroyuki

  Department of Internal Medicine, General Sagami Kosei Hospital

• Maehata Tadateru

  Division of Gastroenterology and Hepatology, Department of Internal Medicine, St. Marianna University School of Medicine

• Suzuki Hiromu

  Department of Molecular Biology, Sapporo Medical University

• Toyota Minoru

  Department of Molecular Biology, Sapporo Medical University

• Itoh Fumio

  Division of Gastroenterology and Hepatology, Department of Internal Medicine, St. Marianna University School of Medicine

Bibliographic Information

Other Title

• マイクロアレイを用いた消化管がんの網羅的メチル化解析

Abstract

Gastrointestinal cancer (GI Cancer) is the leading cause of cancer death in the world. Its prognosis is determined by clinical staging at diagnosis and treatment. Diagnostic tool such as gastrointestinal endoscopy followed by pathological analysis and/or fluoroscopy have proven useful; however, the mortality rate has remained high throughout the world. The need for less invasive and more efficient diagnostic tools has led to a search for GI cancer antigens. Molecular markers that distinguish benign from clinically silent malignant disease are needed to reduce the number of unnecessary endoscopic biopsies and to improve detection of GI cancer at an early stage. Cytosine DNA methylation is an important epigenetic change which leads to the recruitment of transcription repressors and chromatin changes. During the development and progression of GI cancer, many genes are silenced by aberrant methylation of CpG islands, which are CpG dinucleotide-rich areas located within the promoters of approximately 60% of human genes. Recently, methylated CpG island amplification microarray (MCAM) based genome-wide DNA methylation profiles have been available for analyzing primary neoplasms. We reported that DNA methylation analysis using MCAM is useful for the detection of primary GI cancers.

Journal

• SEIBUTSU BUTSURI KAGAKU

  SEIBUTSU BUTSURI KAGAKU 56 (1), 5-8, 2012

  Japanese Electrophoresis Society

Keywords

• GI cancer
• MCAM
• DNA methylation

Details

• CRID: 1390001204202937600
• NII Article ID: 130004456514
• DOI: 10.2198/sbk.56.5
• COI: 1:CAS:528:DC%2BC3sXjtl2hsrw%3D
• ISSN: 13499785
• ISSN: 00319082
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed