Dentin Regeneration by Direct Pulp Capping using rhBMP-2

• YAMAJI Kozo

  Department of Operative Dentistry, Field of Study of Biofunctional Recovery and Reconstruction, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan

• HORIKAWA Gen

  Department of Periodontology and Endodontology, Division of Oral Health Science, Hokkaido University Graduate School of Dental Medicine, Japan

• NISHITANI Yoshihiro

  Department of Operative Dentistry, Field of Study of Biofunctional Recovery and Reconstruction, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan

• IZAWA Shunji

  Department of Operative Dentistry, Field of Study of Biofunctional Recovery and Reconstruction, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan

• YOSHIYAMA Masahiro

  Department of Operative Dentistry, Field of Study of Biofunctional Recovery and Reconstruction, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan

Abstract

The purpose of this study was to evaluate the effect of direct pulp capping using recombinant human BMP-2 (rhBMP-2) on dentin regeneration. RhBMP-2 (1.0 mg/ml) was dissolved in alginate-based gel (AG). Cavities with the exposed pulp area were prepared of first molars in Wister rats. All cavities were divided into two groups: The exposed pulps were covered with AG contained rhBMP-2 (BMP group), and covered with AG alone (AG group). Then they were sealed with glass ionomer cement (Fuji IILC EM). The animals were sacrificed at 3 and 8 weeks after operation. In both groups, the covered materials were observed at 3 weeks after operation, while they had disappeared and the tertiary dentin formation was observed into the prepared cavity at 8 weeks after operation. Thickness of the tertiary dentin of BMP group was significantly higher than that of AG group (P<0.05). These results suggest that AG is effective scaffold and direct pulp capping using AG contains rhBMP-2 is capable of enhancing dentin regeneration.

Journal

• Journal of Oral Tissue Engineering

  Journal of Oral Tissue Engineering 5 (3), 145-149, 2008

  Japanese Association of Regenerative Dentistry

Keywords

• dentin regeneration
• rhBMP-2
• pulp capping

Details

• CRID: 1390001205225620352
• NII Article ID: 110006665907
• NII Article ID: 130004456598
• NII Book ID: AA1195240X
• DOI: 10.11223/jarde.5.145
• ISSN: 18800823
• ISSN: 13489623
• Web Site: https://search.jamas.or.jp/link/ui/2008280183
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed