Sonic Hedgehog Positively Regulates Odontoblast Differentiation by a BMP2/4-dependent Mechanism
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- SHIMO Tsuyoshi
- Department of Oral and Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
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- KOYAMA Eiki
- Department of Orthopaedic Surgery, Thomas Jefferson University College of Medicine, Philadelphia, PA 19107, USA
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- KANYAMA Manabu
- Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
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- KURIO Naito
- Department of Oral and Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
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- OKUI Tatsuo
- Department of Oral and Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
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- YAMAMOTO Daisuke
- Department of Oral and Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
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- HASSAN Nur Mohammad Monsur
- Department of Oral and Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
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- SASAKI Akira
- Department of Oral and Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
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抄録
Sonic hedgehog (Shh) is a cytokine which has important roles in cell differentiation and is believed to interact with other signaling molecules including bone morphogenetic proteins (BMPs). Given that tooth development involves reciprocal epithelial-mesenchymal interactions and cytodifferentiation processes, we asked whether Shh regulates odontoblast differentiation along with BMPs. Shh mRNA was produced by inner dental epithelium; the expression was not constant but varied with development and cytodifferentiation of ameloblasts along the cusp-to-cervix axis. Shh protein (SHH), but not RNA, was present in the underlying differentiating preodontoblasts, probably resulting from gradual diffusion from the epithelial layers. Patched-1, one of hedgehog receptors, was expressed in dental mesenchyme, reflecting paracrine loops of action. Interestingly, BMPs were also expressed in both preameloblasts and preodontoblasts, indicating functional interactions between the two signaling molecules during their differentiation. A new culture system has been established and the process of odontoblast differentiation were analyzed. SHH upregulated the expression levels of odontoblast differentiation markers including Alp and Osteopontin. The stimulatory effect of SHH on ALPase activity was synergistically enhanced by BMP2 or BMP4. Taken together, our data provide clear evidence that SHH is synthesized by dental epithelial layers, reaches the underlying dental mesenchyme, and appears to act as a paracrine factor which regulates odontoblast differentiation in concerted fashion with BMPs.
収録刊行物
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- Journal of Oral Tissue Engineering
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Journal of Oral Tissue Engineering 7 (1), 26-37, 2009
日本再生歯科医学会
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詳細情報 詳細情報について
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- CRID
- 1390001205226525440
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- NII論文ID
- 130004456601
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- ISSN
- 18800823
- 13489623
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可