Cell Viability and Tissue Response of High Molecule Weight DNA/protamine Complex

DOI
  • MORI Nana
    Department of Odontology, Periodontology section, Fukuoka Dental College
  • IWAHASHI Teruaki
    Department of Morphological Biology, Pathology section, Fukuoka Dental College
  • OHNO Jun
    Department of Morphological Biology, Pathology section, Fukuoka Dental College
  • SHINOZAKI Yosuke
    Department of Oral Rehabilitation, Oral Implantology section, Fukuoka Dental College
  • SAKAGAMI Ryuji
    Department of Odontology, Periodontology section, Fukuoka Dental College
  • MITARAI Makoto
    Central Research Institute, Maruha nichiro holdings, Inc.
  • FUKUSHIMA Tadao
    Department of Dental Engineering, Bioengineering section, Fukuoka Dental College

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抄録

The DNA/protamine complex was prepared from the reaction of high molecule weight DNA and protamine. The obtained high molecule weight DNA/protamine complex was water-insoluble white powder. The yield was 75%. The molar binding ratio (molar of protamine/ 1 base pair of DNA) DNA to protamine in was 0.129. SEM observation revealed that prepared complex had a porous structure. The porous disk could be easily prepared. In cell culture tests using MC 3T3-E1 mouse osteoblast cells, cell viabilities for the DNA/protamine complex were from 87.1% to 99.0%. In vivo test, complex showed a mild tissue response after the implantation into the back of skin of rats. The complex was gradually biodegraded during implantation, and a part of complex resided 30 days after implantation. These results suggested that high molecule weight DNA do not influence cytotoxicity and tissue response of DNA/protamine complex.

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