Development of a Novel ELISA for Detection of Anti-A and Anti-B Antibodies in Recipients of ABO-Incompatible Living Donor Liver Grafts

• Satoh Akefumi

  Advanced Surgical Science and Technology, Tohoku University

• Kawagishi Naoki

  Advanced Surgical Science and Technology, Tohoku University

• Minegishi Masayoshi

  Department of Blood Transfusion, Tohoku University Hospital

• Takahashi Hiroyuki

  Department of Blood Transfusion, Tohoku University Hospital

• Akamatsu Yorihiro

  Advanced Surgical Science and Technology, Tohoku University

• Doi Hideyuki

  Advanced Surgical Science and Technology, Tohoku University

• Satomi Susumu

  Advanced Surgical Science and Technology, Tohoku University

Abstract

The survival rate in ABO-incompatible (ABO-I) liver transplantation was much lower than that in ABO-compatible recipients for the early experiences. It is therefore essential to develop the precise and fast measurement of anti-A and anti-B antibodies (Abs) to prevent humoral rejection in ABO-I liver transplantation. Agglutination titer has been the standard method to measure these Abs, but the interpretation of the results is subject to bias. Here, we have developed an objective and quantitative enzyme-linked immunosorbent assay (ELISA) to measure anti-A and anti-B Abs. This test requires only a small amount (10 μl) of recipient's serum. We applied the newly developed ELISA to monitor living donor liver transplant recipients and investigated the correlation between ELISA and agglutination titer. The Spearman's correlation coefficient for Abs ranged from 0.461 to 0.812. Moreover, in one case of humoral rejection, the increase of Abs was detected by ELISA one day earlier than by the agglutination titer. In conclusion, our ELISA method proved useful to detect an increase of anti-A and anti-B Abs titers at an early stage, thereby contributing to a prompt treatment of humoral rejection due to ABO-I.

Journal

• The Tohoku Journal of Experimental Medicine

  The Tohoku Journal of Experimental Medicine 211 (4), 359-367, 2007

  Tohoku University Medical Press

Keywords

• ABO-incompatibility
• ELISA
• living donor liver transplantation
• ABO histo-blood group antibodies
• rejection

Details

• CRID: 1390282679217465472
• NII Article ID: 130004459555
• DOI: 10.1620/tjem.211.359
• ISSN: 13493329
• ISSN: 00408727
• Web Site: http://www.jstage.jst.go.jp/article/tjem/211/4/211_4_359/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007282070
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed