The Uterus Sustains Stable Biological Clock during Pregnancy

• Akiyama Shizuko

  Center for Perinatal Medicine, Tohoku University Hospital Department of Pediatrics, Tohoku University Hospital

• Ohta Hidenobu

  Center for Perinatal Medicine, Tohoku University Hospital Department of Pediatrics, Tohoku University Hospital Department of Obstetrics and Gynecology, Tohoku University Hospital

• Watanabe Shimpei

  Center for Perinatal Medicine, Tohoku University Hospital Department of Pediatrics, Tohoku University Hospital

• Moriya Takahiro

  Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University

• Hariu Aya

  Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University

• Nakahata Norimichi

  Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University

• Chisaka Hiroshi

  Center for Perinatal Medicine, Tohoku University Hospital Department of Obstetrics and Gynecology, Tohoku University Hospital

• Matsuda Tadashi

  Center for Perinatal Medicine, Tohoku University Hospital Department of Pediatrics, Tohoku University Hospital

• Kimura Yoshitaka

  Tohoku University Institute for International Advanced Research and Education

• Tsuchiya Shigeru

  Department of Obstetrics and Gynecology, Tohoku University Hospital

• Tei Hajime

  Kanazawa University Institute of Science and Engineering Faculty of Natural System

• Okamura Kunihiro

  Center for Perinatal Medicine, Tohoku University Hospital Department of Obstetrics and Gynecology, Tohoku University Hospital

• Yaegashi Nobuo

  Center for Perinatal Medicine, Tohoku University Hospital Department of Obstetrics and Gynecology, Tohoku University Hospital

抄録

Maternal circadian information has been reported to play an important role in fetal physiology and development. Hormones and nutrition have been mainly investigated as circadian cues from mother to fetus. However, the influences of circadian properties of the pregnant reproductive organs on fetuses have not been fully investigated. To gain an insight on the circadian functions of the reproductive organs, we examined molecular clocks in the pregnant rat uterus and placenta. By using a Period1-luciferase (Per1-luc) rat, whose tissues express luciferase corresponding to activation of Period1, a “key clock gene”, we examined the uterus clock during non-pregnancy, on embryonic day 12 (E12), and on E22 (the end of pregnancy) in a light-dark (LD) cycle and constant darkness (DD). By in situ hybridization we further explored Per1 mRNA rhythms in the placenta on E12 and E22. The uterus in vitro showed clear circadian Per1-luc rhythms both in and out of pregnancy, having peaks at around the time corresponding to dusk in LD. Likewise, in DD, the uterus in vitro had the same Per1-luc rhythms. The decidua in LD showed circadian Per1 mRNA rhythms, peaking during night 6 h after dusk, while the decidua in DD showed the same Per1 mRNA rhythms only on E22. In contrast, the labyrinth showed no circadian Per1 mRNA rhythms in LD or DD during pregnancy. These results suggest that the uterus and decidua, a maternally-originated tissue of the placenta, but not the labyrinth, a fetus-originated tissue of the placenta, can provide the fetus with circadian information.

収録刊行物

• The Tohoku Journal of Experimental Medicine

  The Tohoku Journal of Experimental Medicine 221 (4), 287-298, 2010

  東北ジャーナル刊行会

キーワード

• intrauterine environments
• circadian
• pregnancy
• uterus
• placenta

詳細情報

• CRID: 1390282679221481600
• NII論文ID: 130004459846
• NII論文ID: 10027085356
• NII書誌ID: AA00863920
• DOI: 10.1620/tjem.221.287
• ISSN: 13493329
• ISSN: 00408727
• Web Site: http://www.jstage.jst.go.jp/article/tjem/221/4/221_4_287/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2011064924
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可