Inheritance of C<SUB>3</SUB>-C<SUB>4</SUB> Intermediate Photosynthesis in Reciprocal Hybrids between <I>Moricandia arvensis</I> (C<SUB>3</SUB>-C<SUB>4</SUB>) and <I>Brassica oleracea</I> (C<SUB>3</SUB>) that Differ in their Genome Constitution

• Ueno Osamu

  Photobiology and Photosynthesis Research Unit, National Institute of Agrobiological Sciences

• Woo Bang Sang

  Faculty of Agriculture, Utsunomiya University

• Wada Yoshiharu

  Faculty of Agriculture, Utsunomiya University

• Kobayashi Nanae

  Faculty of Agriculture, Utsunomiya University

• Kaneko Ryouhei

  Faculty of Agriculture, Utsunomiya University

• Kaneko Yukio

  Faculty of Agriculture, Utsunomiya University

• Matsuzawa Yasuo

  Faculty of Agriculture, Utsunomiya University

Abstract

To elucidate the genetic mechanisms underlying C₃-C₄ intermediate photosynthesis, we investigated the structural and photosynthetic characteristics of leaves of reciprocal hybrids between the C₃-C₄ intermediate species Moricandia arvensis (L.) DC. (MaMa) and the C₃ species Brassica oleracea L. (cabbage; CC), which differ in genome constitution. Moricandia arvensis bundle sheath (BS) cells included many centripetally located chloroplasts and mitochondria, whereas those of cabbage had few organelles. Hybrid leaves were structurally intermediate between those of the parents and showed stronger intermediate C₃-C₄ features as the proportion of the Ma genome increased. The P-protein of glycine decarboxylase (GDC) was confined mainly to BS mitochondria in M. arvensis, but accumulated more in the mesophyll (M) of cabbage. In the hybrids, the accumulation of GDC in BS cells increased with an increasing Ma:C ratio. Hybrids exhibited gradients in structural and biochemical features, even in reciprocal crosses. The CO₂ compensation point of reciprocal hybrids with high Ma:C ratios was lower than that of cabbage but higher than that of M. arvensis. Thus, the structural and biochemical features in hybrid leaves reduced photorespiration. Moricandia arvensis had a higher photosynthetic rate than cabbage, but the photosynthetic rates of hybrids were intermediate between those of the parents or comparable to that of M. arvensis. Our results demonstrate that the C₃-C₄ intermediate characteristics are inherited based on the ratio of the parent genomes, and that there is no evidence of cytoplasmic inheritance in these characteristics.

Journal

• Plant Production Science

  Plant Production Science 10 (1), 68-79, 2007

  CROP SCIENCE SOCIETY OF JAPAN

Keywords

• Brassicaceae
• C<SUB>3</SUB> species
• C<SUB>3</SUB>-C<SUB>4</SUB> intermediate species
• CO<SUB>2</SUB> exchange
• Glycine decarboxylase
• Leaf anatomy
• Photorespiration
• Reciprocal hybrids

Details

• CRID: 1390282679443220224
• NII Article ID: 130004462031
• DOI: 10.1626/pps.10.68
• ISSN: 13491008
• ISSN: 1343943X
• Web Site: https://www.tandfonline.com/doi/pdf/10.1626/pps.10.68
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed