Identification of Two Plastid-Targeted .BETA.-Amylases in Rice

• Hirano Tatsuya

  Graduate School of Agricultural Science, Meijo University

• Takahashi Yusuke

  Graduate School of Agricultural Science, Meijo University

• Fukayama Hiroshi

  Graduate School of Agricultural Science, Kobe University

• Michiyama Hiroyasu

  Graduate School of Agricultural Science, Meijo University

Bibliographic Information

Other Title

• Identification of Two Plastid-Targeted<i>β</i>-Amylases in Rice
• Identification of two plastid-targeted β-amylases in rice

Abstract

In order to identify β-amylase isoforms acting inside plastids in rice (Oryza sativa L.), in the rice genome database we searched the genes predicted to encode β-amylase-like proteins, and designated them OsBAM1−9. OsBAM1, OsBAM7, and OsBAM9 genes were mainly expressed in germinating seeds and developing caryopses. In contrast, the transcripts of OsBAM2, OsBAM3, OsBAM4 and OsBAM5, which have a putative chloroplast transit peptide in their N-terminal regions, were mainly detected in leaf blades, leaf sheaths and internodes after heading. Soluble protein fractions prepared from Escherichia coli containing recombinant OsBAM2 and OsBAM3 proteins had significant β-amylase activity. Furthermore, OsBAM2-GFP and OsBAM3-GFP fusion proteins introduced into epidermis of Allium cepa scaly bulb were localized to the plastids. These results strongly suggest that OsBAM2 and OsBAM3 are plastid-targeted active β-amylase.

Journal

• Plant Production Science

  Plant Production Science 14 (4), 318-324, 2011

  CROP SCIENCE SOCIETY OF JAPAN

Keywords

• β-Amylase
• <i>Oryza sativa</i> L
• Starch metabolism

Details

• CRID: 1390001204468222720
• NII Article ID: 130004462240
• DOI: 10.1626/pps.14.318
• ISSN: 13491008
• ISSN: 1343943X
• Web Site: http://www.tandfonline.com/doi/pdf/10.1626/pps.14.318
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed