Photochemical ODN Manipulation Based on Reversible DNA Photoligation Mediated by Modified Photoresponsive Base

• Ogino Masayuki

  The School of Materials Science, Japan Advanced Institute of Science and Technology

• Ogasawa Shinji

  The School of Materials Science, Japan Advanced Institute of Science and Technology

• Ami Takehiro

  The School of Materials Science, Japan Advanced Institute of Science and Technology

• Yoshimura Yoshinaga

  PRESTO, Japan Science and Technology Agency

• Fujimoto Kenzo

  The School of Materials Science, Japan Advanced Institute of Science and Technology PRESTO, Japan Science and Technology Agency

Abstract

We describe an efficient template-directed photoligation of oligodeoxynucleotides (ODNs) using modified photoresponsive base. An efficient photoligation was produced by photoirradiation of an ODN containing α-5-cyanovinyldeoxyuridine (α^CU) at the 3' end with an ODN containing thymine at the 5' end in the presence of a template ODN. This photoligation method is a new, efficient way to synthesize branched ODNs

Journal

• Journal of Photopolymer Science and Technology

  Journal of Photopolymer Science and Technology 18 (4), 507-512, 2005

  The Society of Photopolymer Science and Technology(SPST)

Keywords

• photoligation
• branched oligodeoxynucleotides
• modified base

Details

• CRID: 1390282679301515648
• NII Article ID: 130004464445
• NII Article ID: 40006743932
• NII Book ID: AA11576862
• DOI: 10.2494/photopolymer.18.507
• ISSN: 13496336
• ISSN: 09149244
• NDL BIB ID: 7338464
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7338464
• Web Site: https://www.jstage.jst.go.jp/article/photopolymer/18/4/18_4_507/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed