Preparation of PEG and Protein Co-immobilized Upconversion Nanophosphors as Near-infrared Biolabeling Materials

• Kamimura Masao

  Graduate School of Pure and Applied Sciences, University of Tsukuba

• Miyamoto Daisuke

  Graduate School of Pure and Applied Sciences, University of Tsukuba sukuba Research Center for Interdisciplinary Materials Sciences (TIMS), University of Tsukuba Center for Tsukuba Advanced Research Alliance (TARA), University of Tsukuba

• Saito Yu

  Department of Materials Science and Technology, Tokyo University of Science

• Soga Kohei

  Center for Tsukuba Advanced Research Alliance (TARA), University of Tsukuba Department of Materials Science and Technology, Tokyo University of Science Polyscale Technology Research Center (PTRC), Tokyo University of Science

• Nagasaki Yukio

  Graduate School of Pure and Applied Sciences, University of Tsukuba sukuba Research Center for Interdisciplinary Materials Sciences (TIMS), University of Tsukuba Center for Tsukuba Advanced Research Alliance (TARA), University of Tsukuba Polyscale Technology Research Center (PTRC), Tokyo University of Science Master's School of Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba Satellite Laboratory, International Center for Materials Nanoarchitectonics (MANA), National Institute of Materials Science (NIMS)

Abstract

Upconversion phosphors are expected to be used as fluorescence biolabeling materials. One of upconversion phosphors, erbium ion-doped-Y₂O₃ (Y₂O₃:Er) nanoparticles (YNPs) was synthesized by homogeneous precipitation method. Poly(ethylene glycol)-b-Poly(acrylic acid) (PEG-b-PAAc) was immobilized on the surface of YNP to improve dispersing stability and prevention of nonspecific adsorption. In order to install protein on the YNP surface, co-immobilization of PEG-b-PAAc and bovine serum albumin (BSA) as a model protein was carried out. The specific molecular recognition of PEG/BSA co-immobilized YNPs (PEG/BSA-YNPs) was confirmed by anti-BSA antibody plate assay. The obtained complex is a promising near-infrared biolabeling materials with high performances.

Journal

• Journal of Photopolymer Science and Technology

  Journal of Photopolymer Science and Technology 21 (2), 183-187, 2008

  The Society of Photopolymer Science and Technology(SPST)

Keywords

• upconversion
• bio-imaging
• inorganic-polymer composite
• nanoparticles
• PEG

Details

• CRID: 1390001204324256768
• NII Article ID: 130004464618
• NII Article ID: 40016108963
• NII Book ID: AA11576862
• DOI: 10.2494/photopolymer.21.183
• COI: 1:CAS:528:DC%2BD1cXosVKrurs%3D
• ISSN: 13496336
• ISSN: 09149244
• NDL BIB ID: 9548740
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9548740
• Web Site: http://www.jstage.jst.go.jp/article/photopolymer/21/2/21_2_183/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed