Amino acid alterations in Gag that confer the ability to grow in simian cells on HIV-1 are located at a narrow CA region

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  • Nagao Tamiko
    Department of Virology, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Hatcho Kazuki
    Department of Virology, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Doi Naoya
    Department of Virology, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Fujiwara Sachi
    Department of Virology, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Adachi Akio
    Department of Virology, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Nomaguchi Masako
    Department of Virology, Institute of Health Biosciences, the University of Tokushima Graduate School

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Abstract

We previously generated a prototype monkey-tropic human immunodeficiency virus type 1 (HIV-1) designated NL-DT5R. This viral clone has a small region of simian immunodeficiency virus (SIV) within Gag capsid (CA) protein and also SIV Vif protein, but displays a poor growth phenotype in simian cells. To improve the growth potential of NL-DT5R, we have constructed a series of its gag variant viruses. Out of fourteen viral clones generated, five were infectious for simian HSC-F cells, and two of the infectious variants grew similarly with NL-DT5R. Taking their genome structures into consideration, our data here clearly show that a narrow CA region within the Gag protein, i.e., the domain around cyclophilin A (CypA)-binding loop, is critical for the growth ability of HIV-1 in simian cells. J. Med. Invest. 56: 21-25, February, 2009

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