Polyphenols prevent clinorotation-induced expression of atrogenes in mouse C2C12 skeletal myotubes

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Author(s)

    • Terao Junji
    • Department of Food Science, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Nikawa Takeshi
    • Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Hirasaka Katsuya
    • Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Nakao Reiko
    • Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Kohno Shohei
    • Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Kagawa Sachiko
    • Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Abe Tomoki
    • Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Harada-Sukeno Akiko
    • Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Okumura Yuushi
    • Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Nakaya Yutaka
    • Department of Nutritional Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School

Abstract

Oxidative stress is a key factor in stimulating the expression of atrogenes, which are muscle atrophy-related ubiquitin ligases, in skeletal muscle, and it induces muscle atrophy during unloading. However, the effects of antioxidative nutrients on atrogene expression have not been demonstrated. We report on the inhibitory effects of polyphenols, such as epicatechin (EC), epicatechin gallate (ECg) and epigallocatechin gallate (EGCg) and quercetin, on atrogene expression up-regulated by three dimensional (3D)-clinorotation or glucocorticoid. These treatments markedly elevated the expression of atrogenes, including atrogin-1 and MuRF-1, in mouse C2C12 myoblasts and myotubes. Interestingly, EC, ECg, EGCg and quercetin significantly decreased the expression of atrogin-1 and MuRF-1 up-regulated by 3D-clinorotation, whereas they hardly affected atrogene expression induced by dexamethasone. ERK signaling is a well known MAPK pathway to mediate oxidative stress. Therefore, we also investigated the effect of these polyphenols on phosphorylation of ERK in C2C12 myotubes. As expected, EC, ECg, EGCg, and quercetin significantly suppressed phosphorylation of ERK, corresponding to the up-regulation of atrogenes induced by 3D-clinorotation. These results suggest that antioxidative nutrients, such as catechins and quercetin, suppress atrogene expression in skeletal muscle cells, possibly through the inhibition of ERK signaling. Thus, catechins and quercetin may prevent unloading-mediated muscle atrophy. J. Med. Invest. 56: 26-32, February, 2009

Journal

  • The Journal of Medical Investigation

    The Journal of Medical Investigation 56(1,2), 26-32, 2009

    The University of Tokushima Faculty of Medicine

Cited by:  2

Codes

  • NII Article ID (NAID)
    130004465163
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    1343-1420
  • Data Source
    CJPref  J-STAGE 
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