Identification and functional analysis of a splice variant of mouse sodium-dependent phosphate transporter Npt2c

DOI IR Web Site PubMed 2 Citations 48 References
  • Kuwahara Shoji
    Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
  • Aranami Fumito
    Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
  • Segawa Hiroko
    Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
  • Onitsuka Akemi
    Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
  • Honda Naoko
    Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
  • Tominaga Rieko
    Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
  • Hanabusa Etsuyo
    Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
  • Kaneko Ichiro
    Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
  • Yamanaka Setsuko
    Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
  • Sasaki Shohei
    Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
  • Ohi Akiko
    Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
  • Nomura Kengo
    Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
  • Tatsumi Sawako
    Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
  • Kido Shinsuke
    Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
  • Ito Mikiko
    Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School University of Hyogo School of Human Science and Environment
  • Miyamoto Ken-ichi
    Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School

Search this article

Abstract

Mutations in the SLC34A3 gene, a sodium-dependent inorganic phosphate (Pi) cotransporter, also referred to as NaPi IIc, causes hereditary hypophosphatemic rickets with hypercalciuria (HHRH), an autosomal recessive disorder. In human and rodent, NaPi IIc is mainly localized in the apical membrane of renal proximal tubular cells. In this study, we identified mouse NaPi IIc variant (Npt2c-v1) that lacks the part of the exon 3 sequence that includes the assumed translation initiation site of Npt2c. Microinjection of mouse Npt2c-v1 cRNA into Xenopus oocytes demonstrated that Npt2c-v1 showed sodium-dependent Pi cotransport activity. The characterization of pH dependency showed activation at extracellular alkaline-pH. Furthermore, Npt2c-v1 mediated Pi transport activity was significantly higher at any pH value than those of Npt2c. In an in vitro study, the localization of the Npt2c-v1 protein was detected in the apical membrane in opossum kidney cells. The expression of Npt2c-v1 mRNA was detected in the heart, spleen, testis, uterus, placenta, femur, cerebellum, hippocampus, diencephalon and brain stem of mouse. Using mouse bone primary cultured cells, we showed the expression of Npt2c-v1 mRNA. In addition, the Npt2c protein was detected in the spermatozoa head. Thus, Npt2c-v1 was expressed in extra-renal tissues such as epididymal spermatozoa and may function as a sodium-dependent phosphate transporter. J. Med. Invest. 59: 116-126, February, 2012

Journal

Citations (2)*help

See more

References(48)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top