光線力学的療法で発生する一重項酸素の計測

• 平野 達

  浜松医科大学光量子医学研究センター

書誌事項

タイトル別名

• Measurement of Singlet Oxygen Generated in Photodynamic Therapy (PDT)

抄録

PDT is based on the generation of singlet oxygen (¹O₂) in a photochemical reaction among a tumor-accumulated photosensitizer, irradiation light, and oxygen in a tumor, and the detection of ¹O₂ is very important for understanding the mechanism of PDT. We used a near-infrared detection system to detect a 1270nm emission that was derived from the ¹O₂. Utilizing this system, we detected the generation of ¹O₂ from Photofrin dissolved in water or injected in a HeLa tumor-bearing mouse. We showed the irradiation power dependence of the generation of ¹O₂ in 5-ALA-mediated PDT with 9L cells and 9L tumor-bearing rats. High-power irradiation reduced the generation of ¹O₂ more than that with low power, and an MTT assay revealed that more 9L cells were killed by low-powered irradiation. In tumors, low-powered irradiation resulted in efficient tumor necrosis. Generations of ¹O₂ in ICG and TiO₂ irradiation are also described.

収録刊行物

• レーザー研究

  レーザー研究 35 (8), 486-492, 2007

  一般社団法人 レーザー学会

キーワード

• Singlet oxygen
• Triplet oxygen
• Photodynamic therapy (PDT)
• Photomultiplier tube
• Transient absorption

詳細情報

• CRID: 1390001204647178496
• NII論文ID: 130004465790
• DOI: 10.2184/lsj.35.486
• COI: 1:CAS:528:DC%2BD2sXhtFSgtbzM
• ISSN: 13496603
• ISSN: 03870200
• Web Site: https://www.jstage.jst.go.jp/article/lsj/35/8/35_486/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可