Hypotaurine is an Energy-Saving Hepatoprotective Compound against Ischemia-Reperfusion Injury of the Rat Liver

• Sakuragawa Tadayuki

  Department of Biochemistry and Integrative Medical Biology, School of Medicine, Keio University

• Hishiki Takako

  Department of Biochemistry and Integrative Medical Biology, School of Medicine, Keio University

• Ueno Yuki

  Department of Biochemistry and Integrative Medical Biology, School of Medicine, Keio University

• Ikeda Satsuki

  Department of Biochemistry and Integrative Medical Biology, School of Medicine, Keio University

• Soga Tomoyoshi

  Institute for Advanced Biosciences, Keio University

• Yachie-Kinoshita Ayako

  Department of Biochemistry and Integrative Medical Biology, School of Medicine, Keio University

• Kajimura Mayumi

  Department of Biochemistry and Integrative Medical Biology, School of Medicine, Keio University

• Suematsu Makoto

  Department of Biochemistry and Integrative Medical Biology, School of Medicine, Keio University

抄録

Metabolome analyses assisted by capillary electrophoresis-mass spectrometry (CE-MS) have allowed us to systematically grasp changes in small molecular metabolites under disease conditions. We applied CE-MS to mine out biomarkers in hepatic ischemia-reperfusion. Rat livers were exposed to ischemia by clamping of the portal inlet followed by reperfusion. Metabolomic profiling revealed that l contents of taurine in liver and plasma were significantly increased. Of interest is an elevation of hypotaurine, collectively suggesting significance of hypotaurine/taurine in post-ischemic responses. Considering the anti-oxidative capacity of hypotaurine, we examined if supplementation of the compound or its precursor amino acids could affect hepatocellular viability and contents of taurine in liver and plasma. Administration of hypotaurine, N-acetylcysteine or methionine upon reperfusion comparablly attenuated the post-ischemic hepatocellular injury but with different metabolomic profiling among groups: rats treated with methionine or N-acetylcysteine but not those treated with hypotaurine, exhibited significant elevation of hepatic lactate generation without notable recovery of the energy charge. Furthermore, the group treated with hypotaurine exhibited elevation of the plasma taurine, suggesting that the exogenously administered compound was utilized as an antioxidant. These results suggest that taurine serves as a surrogate marker for ischemia-reperfusion indicating effectiveness of hypotaurine as an energy-saving hepatoprotective amino acid.<br>

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ 46 (2), 126-134, 2010

  一般社団法人　日本酸化ストレス学会

キーワード

• metabolome
• CE-MS
• hypotaurine
• taurine
• reperfusion injury

詳細情報

• CRID: 1390001204672655360
• NII論文ID: 130004466603
• DOI: 10.3164/jcbn.09-91
• ISSN: 18805086
• ISSN: 09120009
• Web Site: https://search.jamas.or.jp/link/ui/2010238150
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可