Direct assessment by electron spin resonance spectroscopy of the antioxidant effects of French maritime pine bark extract in the maxillofacial region of hairless mice

• Yoshida Ayaka

  Department of Clinical Care Medicine, Division of Pharmacology and ESR Laboratories, Kanagawa Dental College

• Yoshino Fumihiko

  Department of Clinical Care Medicine, Division of Pharmacology and ESR Laboratories, Kanagawa Dental College

• Tsubata Masahito

  Toyo Shinyaku Co., Ltd., Research and Development Division

• Ikeguchi Motoya

  Toyo Shinyaku Co., Ltd., Research and Development Division

• Nakamura Takeshi

  Pharmacokinetics and Bioanalysis Center, Research Group 1, Research Department 1, Shin Nippon Biomedical Laboratories, LTD.

• Lee Masaichi-Chang-il

  Department of Clinical Care Medicine, Division of Pharmacology and ESR Laboratories, Kanagawa Dental College

抄録

Flavangenol, one of extract of French maritime pine bark, is a complex mixture of bioflavonoids with oligometric proanthocyanidins as the major constituents. These constituents, catechin and procyanidin B₁, are water-soluble derivatives of flavangenol. In this study, we investigated the antioxidant effects of flavangenol on reactive oxygen species such as hydroxyl radical, superoxide anion and singlet oxygen using electron spin resonance and spin trapping. The effect of flavangenol on oxidative stress in the skin from the maxillofacial region of hairless mice was investigated using an in vivo L-band electron spin resonance imaging system. Flavangenol attenuated oxidative stress in the maxillofacial skin by acting as a reactive oxygen species scavenger, as demonstrated by in vitro and in vivo electron spin resonance imaging analysis. The absorption and metabolism of flavangenol were also examined. After oral administration of flavangenol in human and rat, most of the catechin in plasma was in the conjugated form, while 45% to 78% of procyanidin B₁ was unconjugated, indicating that non-conjugated procyanidin B₁ would be active in the circulation. The ability of flavangenol to reduce reactive oxygen species levels in the circulation of the maxillofacial region suggests that this extract may be beneficial for skin protection from exposure to ultraviolet irradiation.<br>

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ 49 (2), 79-86, 2011

  一般社団法人　日本酸化ストレス学会

キーワード

• reactive oxygen species
• oxidative stress
• electron spin resonance (ESR)
• antioxidants
• French maritime pine bark extract

詳細情報

• CRID: 1390282679648821888
• NII論文ID: 130004466650
• DOI: 10.3164/jcbn.10-103
• ISSN: 18805086
• ISSN: 09120009
• Web Site: https://search.jamas.or.jp/link/ui/2012047875
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可