Crocetin reduces the oxidative stress induced reactive oxygen species in the stroke-prone spontaneously hypertensive rats (SHRSPs) brain

• Yoshino Fumihiko

  Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College

• Yoshida Ayaka

  Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College

• Umigai Naofumi

  Riken Vitamin Co., Ltd.

• Kubo Koya

  Riken Vitamin Co., Ltd.

• Lee Masaichi-Chang-il

  Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College

抄録

Crocetin is a natural carotenoid compound of gardenia fruits and saffron, which has various effects in biological systems. In this study, we investigated the antioxidant effects of crocetin on reactive oxygen species such as hydroxyl radical using in vitro X-band electron spin resonance and spin trapping. Crocetin significantly inhibited hydroxyl radical generation compared with the control. Moreover, we performed electron spin resonance computed tomography ex vivo with the L-band electron spin resonance imaging system and determined the electron spin resonance signal decay rate in the isolated brain of stroke-prone spontaneously hypertensive rats, a high-oxidative stress model. Crocetin significantly reduced oxidative stress in the isolated brain by acting as a scavenger of reactive oxygen species, especially hydroxyl radical, as demonstrated by in vitro and ex vivo electron spin resonance analysis. The distribution of crocetin was also determined in the plasma and the brain of stroke-prone spontaneously hypertensive rats using high-performance liquid chromatography. After oral administration, crocetin was detected at high levels in the plasma and the brain. Our results suggest that crocetin may participate in the prevention of reactive oxygen species-induced disease due to a reduction of oxidative stress induced by reactive oxygen species in the brain.<br>

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ 49 (3), 182-187, 2011

  一般社団法人　日本酸化ストレス学会

キーワード

• crocetin
• antioxidant
• oxidative stress
• brain
• electron spin resonance (ESR)

詳細情報

• CRID: 1390001204673358976
• NII論文ID: 130004466653
• DOI: 10.3164/jcbn.11-01
• ISSN: 18805086
• ISSN: 09120009
• Web Site: https://search.jamas.or.jp/link/ui/2012063110
• 本文言語コード: en
• データソース種別:
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• 抄録ライセンスフラグ: 使用不可