Effect of branched-chain amino acid supplementation on the oxidized/reduced state of plasma albumin in rats with chronic liver disease

• Kuwahata Masashi

  Departments of Nutrition Science, Kyoto Prefectural University

• Kubota Hiroyo

  Departments of Nutrition Science, Kyoto Prefectural University

• Katsukawa Misaki

  Departments of Nutrition Science, Kyoto Prefectural University

• Ito Shunsuke

  Departments of Nutrition Science, Kyoto Prefectural University

• Ogawa Aki

  Departments of Nutrition Science, Kyoto Prefectural University

• Kobayashi Yukiko

  Departments of Nutrition Science, Kyoto Prefectural University

• Nakamura Yasushi

  Food Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University

• Kido Yasuhiro

  Departments of Nutrition Science, Kyoto Prefectural University

Abstract

We examined whether continuous supplementation with branched-chain amino acids phosphorylates ribosomal protein S6, a downstream effector of mammalian target of rapamycin, and improves hypoalbuminemia of rats with chronic liver disease. Sprague-Dawley rats were fed a casein diet (control group) or a branched-chain amino acid-supplemented casein diet (branched-chain amino acid group) for 11 weeks with repeated injections of carbon tetrachloride. Throughout this experimental period, no significant difference in plasma albumin concentration was seen between groups. The percentage of reduced albumin within total plasma albumin gradually decreased in both control and branched-chain amino acid groups. After 11 weeks with supplementation, phosphorylation of ribosomal protein S6 was significantly increased in the liver of rats in the branched-chain amino acid group compared with the control group. Furthermore, the percentage of reduced albumin within total albumin was significantly higher in the branched-chain amino acid group than in the control group. These results indicate that continuous supplementation with branched-chain amino acids in rats with chronic liver disease induces phosphorylation of hepatic ribosomal protein S6 and attenuates decreases in the percentage of reduced albumin, although levels of plasma albumin are not increased.<br>

Journal

• Journal of Clinical Biochemistry and Nutrition

  Journal of Clinical Biochemistry and Nutrition 50 (1), 67-71, 2011

  SOCIETY FOR FREE RADICAL RESEARCH JAPAN

Keywords

• branched-chain amino acids
• chronic liver disease
• reduced albumin
• ribosomal protein S6
• rats

Details

• CRID: 1390001204672900096
• NII Article ID: 130004466672
• DOI: 10.3164/jcbn.11-37
• ISSN: 18805086
• ISSN: 09120009
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed