Analysis of vanin-1 upregulation and lipid accumulation in hepatocytes in response to a high-fat diet and free fatty acids

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Author(s)

    • Motomura Wataru
    • Department of Microbiology and Immunochemistry, Asahikawa Medical College
    • Takahashi Nobuhiko
    • Department of Medicine, Division of Gastroenterology and Hematology/Oncology, Asahikawa Medical College
    • Kumei Shima
    • Department of Medicine, Division of Gastroenterology and Pharmacology, Asahikawa Medical College
    • Mizukami Yusuke
    • Department of Medicine, Division of Gastroenterology and Hematology/Oncology, Asahikawa Medical College
    • Jang Seong-Jae
    • Department of Microbiology and Immunochemistry, Asahikawa Medical College
    • Kohgo Yutaka
    • Department of Medicine, Division of Gastroenterology and Hematology/Oncology, Asahikawa Medical College

Abstract

High-fat diet is one of the causes of nonalcoholic fatty liver disease. We have previously demonstrated that high-fat diet induces upregulation of adipose differentiation-related protein mRNA expression accompanied by lipid droplet formation in mouse liver. Vanin-1 is a ubiquitous epithelial ectoenzyme that has pantetheinase activity and produces cysteamine, a potent endogenous antioxidant. In the present study, we analyzed the expression of hepatic vanin-1 mRNA following the administration of a high-fat diet in mice as well as free fatty acids in hepatocyte cultures and speculated its possible mechanism. Vanin-1 mRNA levels in the livers of mice were upregulated within a day of the high-fat diet, even before the expression of adipose differentiation-related protein mRNA and lipid accumulation. An <i>in vitro</i> analysis using HuH-7 cells revealed a significant upregulation of vanin-1 mRNA by as low as 0.01 mM oleic acid; however, lipid accumulation in hepatocytes was not affected at this concentration. Furthermore, vanin-1 mRNA was differentially upregulated by various free fatty acids irrespective of the grade of lipid accumulation. These findings indicate that the upregulation of vanin-1 precedes lipid accumulation and is differentially mediated by various types of free fatty acids in the model, presenting vanin-1 as a novel player in the pathogenesis of nonalcoholic fatty liver disease.<br>

Journal

  • Journal of Clinical Biochemistry and Nutrition

    Journal of Clinical Biochemistry and Nutrition 51(3), 163-169, 2012

    SOCIETY FOR FREE RADICAL RESEARCH JAPAN

Codes

  • NII Article ID (NAID)
    130004466691
  • Text Lang
    ENG
  • ISSN
    0912-0009
  • Data Source
    J-STAGE 
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