Bactericidal effect of hydroxyl radicals generated from a low concentration hydrogen peroxide with ultrasound in endodontic treatment
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- Kobayashi Yoshimi
- Department of Endodontics, Nihon University School of Dentistry
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- Hayashi Makoto
- Department of Endodontics, Nihon University School of Dentistry
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- Yoshino Fumihiko
- Photomedical Dentistry, Department of Oral Science Graduate School of Kanagawa Dental University
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- Tamura Muneaki
- Department of Microbiology, Nihon University School of Dentistry
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- Yoshida Ayaka
- Photomedical Dentistry, Department of Oral Science Graduate School of Kanagawa Dental University
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- Ibi Haruna
- Department of Endodontics, Nihon University School of Dentistry
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- Lee Masaichi-Chang-il
- Yokosuka-Shonan Disaster Health Emergency Research Center & ESR Laboratories, Kanagawa Dental University
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- Ochiai Kuniyasu
- Department of Microbiology, Nihon University School of Dentistry
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- Ogiso Bunnai
- Department of Endodontics, Nihon University School of Dentistry
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抄録
One approach to enhance the disinfection of root canals in endodontic treatment is ultrasonic irrigation with sodium hypochlorite. Reactive oxygen species, such as hydroxyl radical, are generated by biological defense systems to kill invading bacteria. Ultrasonic irrigation with hydrogen peroxide may be a promising option to increase hydroxyl radical generation. We examined the bactericidal effects of hydroxyl radical generated from low concentration hydrogen peroxide with ultrasound in vitro. An ultrasonic tip was submerged in 0.5 or 1.0 M hydrogen peroxide in a microfuge tube. hydrogen peroxide was irradiated with the ultrasound, the tip of which was maintained centered in the tube to mimic ultrasonic irrigation. Hydroxyl radical generation was assessed by electron spin resonance spectroscopy. Subsequently, Enterococcus faecalis suspension in hydrogen peroxide was prepared and irradiated as described above. Bactericidal effects were assessed by viable counting. Electron spin resonance measurements showed that hydroxyl radical generation increased significantly in a time- and dose-dependent manner (two-way analysis of variance and Tukey’s test, p<0.05). Moreover, the bactericidal effects of hydrogen peroxide against Enterococcus faecalis were enhanced by ultrasonic irradiation in a time- and dose-dependent manner. These results suggest that ultrasonic irrigation in the presence of low concentration hydrogen peroxide can serve as a disinfection strategy in endodontic treatment.
収録刊行物
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- Ｊｏｕｒｎａｌ ｏｆ Ｃｌｉｎｉｃａｌ Ｂｉｏｃｈｅｍｉｓｔｒｙ ａｎｄ Ｎｕｔｒｉｔｉｏｎ
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Ｊｏｕｒｎａｌ ｏｆ Ｃｌｉｎｉｃａｌ Ｂｉｏｃｈｅｍｉｓｔｒｙ ａｎｄ Ｎｕｔｒｉｔｉｏｎ 54 (3), 161-165, 2014
一般社団法人 日本酸化ストレス学会