Lymphatic distribution in the mouse periodontal ligament

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  • Masuyama Mikiko
    Department of Orthodontics, School of Dentistry, Iwate Medical University
  • Fujimura Akira
    First Department of Oral Anatomy, School of Dentistry, Iwate Medical University

Abstract

Observing the changes in periodontium due to orthodontic force is important to reveal the mechanisms of tooth movement. In order to understand micro-vascular dynamics in regards to bone remodeling, we considered that lymphatics functioning need to be examined along with the blood vessels. The aims of this study were to reveal the lymphatic distribution in the mouse periodontal ligament using immunohistochemical methods. A cryosection film-transfer method was used to produce 2 µm-thick non-decalcified serial sections, and immunohistochemical staining using antibody to lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) which was demonstrated for lymphatics was performed. The number of LYVE-1 immunoreactive vessels in the periodontal ligament were sparser than that of blood vessels in the periodontal ligament. All LYVE-1 positive lymphatics were observed close to the alveolar bone-wall in the apical periodontal ligament. However, their horizontal anastomoses were not observed. Distribution of LYVE-1 positive lymphatics in an umbrella frame-like was observed at the apical region. These lymphatics and the lymphatics in the tooth pulp merged into collecting lymphatics in the mandibular canal. This sparse distribution of lymphatics suggests a very low absorption capacity in the periodontal ligament. [MVRC 3(1): 2-10, 2009]

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