Histological assessments on the abnormalities of mouse epiphyseal chondrocytes with short term centrifugal loading

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Author(s)

    • de FREITAS Paulo Henrique Luiz
    • Divisions of Oral and Maxillofacial Surgery, Niigata University Graduate School of Medical and Dental Sciences|Center for Transdisciplinary Research, Niigata University
    • KOJIMA Taku
    • Divisions of Reconstructive Surgery for Oral and Maxillofacial Region, Niigata University Graduate School of Medical and Dental Sciences|Center for Transdisciplinary Research, Niigata University
    • UBAIDUS Sobhan
    • Divisions of Oral and Maxillofacial Surgery, Niigata University Graduate School of Medical and Dental Sciences|Center for Transdisciplinary Research, Niigata University
    • LI Minqi
    • Center for Transdisciplinary Research, Niigata University
    • SHANG Guangwei
    • Center for Transdisciplinary Research, Niigata University|Center of Health Care in Stomatology, Shanghai 10th Hospital, Tongji University
    • TAKAGI Ritsuo
    • Divisions of Oral and Maxillofacial Surgery, Niigata University Graduate School of Medical and Dental Sciences
    • MAEDA Takeyasu
    • Divisions of Oral Anatomy, Niigata University Graduate School of Medical and Dental Sciences|Center for Transdisciplinary Research, Niigata University
    • ODA Kimimitsu
    • Divisions of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences|Center for Transdisciplinary Research, Niigata University

Abstract

We have examined the morphological changes in chondrocytes after exposure to experimental hypergravity. Tibial epiphyseal cartilages of 17-days-old mouse fetuses were exposed to centrifugation at 3G for 16 h mimicking hypergravitational environment (experimental group), or subjected to stationary cultures (control group). Centrifugation did not affect the sizes of epiphyseal cartilage, chondrocyte proliferation, type X collagen-positive hypertrophic zone, and the mRNA expressions of parathyroid hormone-related peptide and fibroblast growth factor receptor III. However, centrifuged chondrocytes showed abnormal morphology and aberrant spatial arrangements, resulting in disrupted chondrocytic columns. Through histochemical assessments, actin filaments were shown to distribute evenly along cell membranes of control proliferative chondrocytes, while chondrocytes subjected to centrifugal force developed a thicker layer of actin filaments. Transmission electron microscopic observations revealed spotty electron-dense materials underlying control chondrocytes' cell membranes, while experimental chondrocytes showed their thick layer. In the intracolumnar regions of the control cartilage, longitudinal electron-dense fibrils were associated with short cytoplasmic processes of normal chondrocytes, indicating assumed cell-tomatrix interactions. These extracellular fibrils were disrupted in the centrifuged samples. Summarizing, altered actin filaments associated with cell membranes, irregular cell shape and disappearance of intracolumnar extracellular fibrils suggest that hypergravity disturbs cell-to-matrix interactions in our cartilage model.

Journal

  • Biomedical Research

    Biomedical Research 28(4), 191-203, 2007

    Biomedical Research Press

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