Changes of HCN gene expression and If currents in Nkx2.5-positive cardiomyocytes derived from murine embryonic stem cells during differentiation

• Yano Shuichi

  Department of Cardiovascular Medicine, Tottori University Faculty of Medicine

• Miake Junichiro

  Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science

• Mizuta Einosuke

  Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science

• Manabe Kasumi

  Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science

• Bahrudin Udin

  Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science

• Morikawa Kumi

  Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science

• Arakawa Keita

  Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science

• Sasaki Norihito

  Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science

• Igawa Osamu

  Department of Cardiovascular Medicine, Tottori University Faculty of Medicine

• Shigemasa Chiaki

  Department of Cardiovascular Medicine, Tottori University Faculty of Medicine

• Yamamoto Yasutaka

  Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science

• Morisaki Takayuki

  Department of Bioscience, National Cardiovascular Center Research Institute

• Hidaka Kyoko

  Department of Bioscience, National Cardiovascular Center Research Institute

• Kurata Yasutaka

  Department of Physiology, Kanazawa Medical College

• Yoshida Akio

  Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science

• Shiota Goshi

  Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Tottori University Graduate School of Medical Science

• Higaki Katsumi

  Division of Functional Genomics, Research Center for Bioscience and Technology, Tottori University

• Ninomiya Haruaki

  Department of Biological Regulation, Tottori University Faculty of Medicine

• Lee Jong-Kook

  Department of Cardiovascular Research, Research Institute of Environmental Medicine, Nagoya University

• Shirayoshi Yasuaki

  Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science

• Hisatome Ichiro

  Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science

抄録

Changes in the expression of hyperpolarization-activated cyclic nucleotide (HCN)-gated channels and I_f currents during the differentiation of embryonic stem cells into cardiac cells remain unknown. We examined changes of HCN genes in expression and function during the differentiation of Nkx2.5-positive cardiac precursor cells derived from mouse ES cells using cell sorting, RTPCR, immunofluorescence and whole cell patch-clamp techniques. Cs⁺-induced inhibition of automaticity and transcription of HCN genes increased during differentiation. Expressions of Nkx2.5, a marker of cardiac progenitor cell, and Flk1, a marker of hemangioblast, were mutually exclusive. Messenger RNA and proteins encoded by HCN1 and 4 genes were predominantly observed in Nkx2.5-positive cells on day 15, although Flk1-positive cells did not express genes of the HCN family on that day. Cs⁺-induced prolongation of the cycle of spontaneous action potentials and I_f currents were predominantly observed on day 15. These results suggested that a fraction of Nkx2.5-positive cardiac precursor cells was committed to pacemaking cells expressing I_f channels predominantly encoded by HCN 1 and 4 genes.

収録刊行物

• Biomedical Research

  Biomedical Research 29 (4), 195-203, 2008

  バイオメディカルリサーチプレス

キーワード

• General Biochemistry, Genetics and Molecular Biology
• General Medicine

詳細情報

• CRID: 1390282679877249024
• NII論文ID: 130004470715
• DOI: 10.2220/biomedres.29.195
• COI: 1:CAS:528:DC%2BD1cXhtVSku7rL
• ISSN: 1880313X
• ISSN: 03886107
• Web Site: https://search.jamas.or.jp/link/ui/2009046133
• 本文言語コード: en
• データソース種別:
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  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可