Bone and cartilage repair by transplantation of induced pluripotent stem cells in murine joint defect model

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Author(s)

    • UTO Sakura
    • Department of Cartilage & Bone Regeneration (Fujisoft) Graduate Schoolof Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan|Department of Sensory and Motor System Medicine, Graduate Schoolof Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan
    • NISHIZAWA Satoru
    • Department of Cartilage & Bone Regeneration (Fujisoft) Graduate Schoolof Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan|Translational Research Center, The Universityof Tokyo Hospital, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan
    • TAKASAWA Yutaka
    • Department of Pathology, The Cancer Institute Hospitalof Japanese Foundation for Cancer Research, 3-8-31 Ariake, Koto-ku, Tokyo, 135-8550, Japan
    • ASAWA Yukiyo
    • Department of Cartilage & Bone Regeneration (Fujisoft) Graduate Schoolof Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan
    • FUJIHARA Yuko
    • Department of Cartilage & Bone Regeneration (Fujisoft) Graduate Schoolof Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan|Department of Sensory and Motor System Medicine, Graduate Schoolof Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan
    • TAKATO Tsuyoshi
    • Department of Sensory and Motor System Medicine, Graduate Schoolof Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan
    • HOSHI Kazuto
    • Department of Cartilage & Bone Regeneration (Fujisoft) Graduate Schoolof Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan|Department of Sensory and Motor System Medicine, Graduate Schoolof Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan

Abstract

The establishment of cartilage regenerative medicine has been an important issue in the clinical field, because cartilage has the poor ability of self-repair. Currently, tissue engineering using autologous chondrocytes has risen, but we should investigate more appropriate cell sources that can be obtained without any quantitative limitation. In this study, we focused on induced pluripotent stem (iPS) cells, in which the ethical hurdle does not seem higher than that of embryonic stem cells. Mouse iPS cells were transplanted into the mouse joint defect model of the knee. Strains of the transplants and hosts were arranged to be either closest (homology 75% in genetic background) or identical (100%). For transplantation, we embedded the iPS cells within the collagen hydrogel in order to obtain the effective administration of the cells into defects, which induced the differentiation of the iPS cells. At 8 weeks of transplantation, although the iPS cells with a 75% homology to the host in the genetic background tended to form teratoma, those of 100% showed a joint regeneration. GFP immunohistochemistry proved that the transplanted iPS cells were responsible for the bone and cartilage repair. Taking these results together, the iPS cells are regarded as a promising cell source for the cartilage tissue engineering.

Journal

  • Biomedical Research

    Biomedical Research 34(6), 281-288, 2013

    Biomedical Research Press

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