ST2 Requires Th2-, but Not Th17-, Type Airway Inflammation in Epicutaneously Antigen-Sensitized Mice

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Author(s)

    • Morita Hideaki
    • Department of Pediatrics, Keio University School of Medicine|Department of Allergy and Immunology, National Research Institute for Child Health and Development
    • Matsumoto Kenji
    • Department of Allergy and Immunology, National Research Institute for Child Health and Development
    • Nakae Susumu
    • Department of Allergy and Immunology, National Research Institute for Child Health and Development|Atopy Research Center, Juntendo University School of Medicine|Frontier Research Initiative, The Institute of Medical Science, The University of Tokyo|Laboratory of Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo|Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency
    • Arae Ken
    • Department of Allergy and Immunology, National Research Institute for Child Health and Development|Department of Immunology, Faculty of Health Science, Kyorin University
    • Ohno Tatsukuni
    • Department of Allergy and Immunology, National Research Institute for Child Health and Development
    • Oboki Keisuke
    • Department of Allergy and Immunology, National Research Institute for Child Health and Development
    • Matsuda Akio
    • Department of Allergy and Immunology, National Research Institute for Child Health and Development
    • Suto Hajime
    • Atopy Research Center, Juntendo University School of Medicine
    • Okumura Ko
    • Atopy Research Center, Juntendo University School of Medicine

Abstract

<b>Background:</b> IL-33 is known to induce Th2-type cytokine production by various types of cells through its receptors, ST2 and IL-1RAcP. Polymorphism in the ST2 and/or IL-33 genes was found in patients with atopic dermatitis and asthma, implying that the IL-33/ST2 pathway is closely associated with susceptibility to these diseases. Exposure to allergens through damaged skin is suspected to be a trigger for allergen sensitization, resulting in development of such allergic disorders as asthma and atopic dermatitis.<br> <b>Methods:</b> To elucidate the role(s) of the IL-33/ST2 pathway in asthma in individuals who had been epicutaneously sensitized to an antigen, wild-type and ST2<sup>-/-</sup> mice were epicutaneously sensitized with ovalbumin (OVA) and then were intranasally challenged with OVA. The degree of airway inflammation, the number of leukocytes and the activities of myeloperoxidase (MPO) and eosinophil peroxidase (EPO) in bronchoalveolar lavage fluids (BALFs), The levels of cytokines and chemokines in lungs and OVA-specific IgE levels in sera were determined by histological analysis, a hemocytometer, colorimetric assay, quantitative PCR or ELISA, respectively.<br> <b>Results:</b> The number of eosinophils in BALFs, the levels of Th2 cytokines and chemoattractants in the lungs and OVA-specific IgE in sera from ST2<sup>-/-</sup> mice were significantly reduced compared with wild-type mice. Although the number of neutrophils in BALFs and the pulmonary levels of IL-17 were comparable in both mice, the levels of MPO activity in BALFs and neutrophil chemoattractants in the lung were reduced in ST2<sup>-/-</sup> mice.<br> <b>Conclusions:</b> The IL-33/ST2 pathway is crucial for Th2-cytokine-mediated eosinophilic, rather than Th17-cytokine-mediated neutrophilic, airway inflammation in mice that had been epicutaneously sensitized with antigens and then challenged with antigen.<br>

Journal

  • Allergology International

    Allergology International 61(2), 265-273, 2012

    Japanese Society of Allergology

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