Selective differentiation of bone marrow-derived mesenchymal stromal cells into osteocytes via endochondral ossification in an apatite-fiber scaffold

• HONDA Michiyo

  Aizawa “Next-generation Bioceramics” Project, Kanagawa Academy of Science and Technology (KAST)

• WATANABE Yuka

  Department of Materials and Life sciences, Faculty of Science and Technology, Sophia University

• TSUCHIYA Takahide

  Department of Materials and Life sciences, Faculty of Science and Technology, Sophia University

• KANZAWA Nobuyuki

  Department of Materials and Life sciences, Faculty of Science and Technology, Sophia University

• AIZAWA Mamoru

  Aizawa “Next-generation Bioceramics” Project, Kanagawa Academy of Science and Technology (KAST) Department of Applied Chemistry, School of Science and Technology, Meiji University

Abstract

Bone marrow-derived mesenchymal stromal cells (BMSCs) are pluripotent progenitor cells that can regenerate different skeletal tissues in response to environmental signals. Scaffolds play a critical role in tissue engineering, and their microstructure is essential for inductive bone formation. In the present study, we have used highly porous, structurally stable three-dimensional apatite-fiber scaffolds (AFSs) and investigated their ability to support cell proliferation and differentiation. BMSCs in/on AFSs were proliferated in a three-dimensional manner when both micro- and macro-pores were present. The characteristic structure of the AFS enhanced calcification, production of extracellular matrix (ECM), and osteogenic differentiation, especially endochondral ossification. Furthermore, BMSCs cultured in/on AFSs did not undergo adipogenesis and selectively underwent osteogenic differentiation. These results suggest that AFSs provide a suitable environment for bone regeneration and have potential applications for tissue engineering.

Journal

• Journal of the Ceramic Society of Japan

  Journal of the Ceramic Society of Japan 121 (1417), 759-765, 2013

  The Ceramic Society of Japan

Keywords

• Scaffold
• Hydroxyapatite
• Bone marrow-derived stromal cell
• Endochondral ossification
• Differentiation

Details

• CRID: 1390001205287854592
• NII Article ID: 130004480215
• DOI: 10.2109/jcersj2.121.759
• ISSN: 13486535
• ISSN: 18820743
• Web Site: https://www.jstage.jst.go.jp/article/jcersj2/121/1417/121_JCSJ-P13104/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed